A preliminary assessment of matK, rbcL and trnH-psbA as DNA barcodes for Calamus (Arecaceae) species in China with a note on ITS

CORC > 昆明植物研究所 > 中国科学院昆明植物研究所 > 中国科学院东亚植物多样性与生物地理学重点实验室

	A preliminary assessment of matK, rbcL and trnH-psbA as DNA barcodes for Calamus (Arecaceae) species in China with a note on ITS
	Yang, Han-Qi 1; Dong, Yu-Ran 1; Gu, Zhi-Jia 1; Liang, Ning 1; Yang, Jun-Bo 2
刊名	ANNALES BOTANICI FENNICI
	2012-11-30
卷号	49 期号:5-6 页码:319-330
关键词	MOLECULAR PHYLOGENETICS FLOWERING PLANTS SEQUENCE DATA LAND PLANTS PLASTID DNA IDENTIFICATION PALMAE SUBSTITUTION CALAMOIDEAE TAXONOMY
ISSN号	0003-3847
通讯作者	Yang, HQ (reprint author), Chinese Acad Forestry, Res Inst Resources Insects, Kunming 650224, Yunnan, Peoples R China.,yanghanqikm@yahoo.com.cn
英文摘要	Calamus is the largest genus in the palm family (Arecaceae) and contains many species of high ecological and economical value. In this study, we assessed the nuclear ribosomal internal transcribed spacer region (ITS), matK, rbcL, trnH-psbA, as well as two combinations, matK + rbcL and matK + rbcL + trnH-psbA, as DNA barcodes for Calamus using 15 species or varieties distributed in China. ITS may exist as multiple copies in the examined Calamus species, and was eliminated from consideration as a possible barcode. The trnH-psbA spacer had the most variation, followed by matK and rbcL. No separations between intraspecific variation and interspecific divergence (barcoding gaps) were found in the remaining candidate barcodes. At the species level, the discrimination rates of the candidate barcodes based on neighbor-joining (NJ) trees were significantly different: matK (37.5%), rbcL (6.3%), trnH-psbA (56.3%), matK + rbcL (43.8%) and matK + rbcL + trnH-psbA (62.5%). Furthermore, the discrimination rates of trnH-psbA would improve to 91.7%, if the varieties of C. nambariensis and C. yunnanensis were treated as one species in the NJ tree. Thus, trnH-psbA may be an appropriate single DNA barcode for Calamus useful in the species identification.
学科主题	Plant Sciences
类目[WOS]	Plant Sciences
研究领域[WOS]	Plant Sciences
关键词[WOS]	MOLECULAR PHYLOGENETICS ; FLOWERING PLANTS ; SEQUENCE DATA ; LAND PLANTS ; PLASTID DNA ; IDENTIFICATION ; PALMAE ; SUBSTITUTION ; CALAMOIDEAE ; TAXONOMY
收录类别	SCI
资助信息	State Forestry Administration of China [2008-4-30]; Large-scale Scientific Facilities of the Chinese Academy of Sciences [2009-LSF-GBOWS-01]; Applied Basic Program of Yunnan [2010CD141]
语种	英语
WOS记录号	WOS:000311767300003
公开日期	2013-01-22
内容类型	期刊论文
源URL	[http://ir.kib.ac.cn/handle/151853/15962]
专题	昆明植物研究所_中国科学院东亚植物多样性与生物地理学重点实验室
作者单位	1.Chinese Acad Forestry, Res Inst Resources Insects, Kunming 650224, Yunnan, Peoples R China 2.Chinese Acad Sci, Kunming Inst Bot, Key Lab Biodivers & Biogeog, Germplasm Bank Wild Species, Kunming 650204, Yunnan, Peoples R China
推荐引用方式 GB/T 7714	Yang, Han-Qi,Dong, Yu-Ran,Gu, Zhi-Jia,et al. A preliminary assessment of matK, rbcL and trnH-psbA as DNA barcodes for Calamus (Arecaceae) species in China with a note on ITS[J]. ANNALES BOTANICI FENNICI,2012,49(5-6):319-330.
APA	Yang, Han-Qi,Dong, Yu-Ran,Gu, Zhi-Jia,Liang, Ning,&Yang, Jun-Bo.(2012).A preliminary assessment of matK, rbcL and trnH-psbA as DNA barcodes for Calamus (Arecaceae) species in China with a note on ITS.ANNALES BOTANICI FENNICI,49(5-6),319-330.
MLA	Yang, Han-Qi,et al."A preliminary assessment of matK, rbcL and trnH-psbA as DNA barcodes for Calamus (Arecaceae) species in China with a note on ITS".ANNALES BOTANICI FENNICI 49.5-6(2012):319-330.