Mapping the C-elegans noncoding transcriptome with a whole-genome tiling microarray

doi:10.1101/gr.661807

CORC > 计算技术研究所 > 中国科学院计算技术研究所 > 中国科学院计算技术研究所期刊论文 > 英文

	Mapping the C-elegans noncoding transcriptome with a whole-genome tiling microarray
	He, Housheng ; Wang, Jie ; Liu, Tao ; Liu, X. Shirley ; Li, Tiantian ; Wang, Yunfei ; Qian, Zuwei ; Zheng, Haixia ; Zhu, Xiaopeng ; Wu, Tao
刊名	GENOME RESEARCH
	2007-10-01
卷号	17 期号:10 页码:1471-1477
ISSN号	1088-9051
DOI	10.1101/gr.661807
英文摘要	The number of annotated protein coding genes in the genome of Caenorhabditis elegans is similar to that of other animals, but the extent of its non- protein- coding transcriptome remains unknown. Expression profiling on whole- genome tiling microarrays applied to a mixed- stage C. elegans population verified the expression of 71% of all annotated exons. Only a small fraction ( 11%) of the polyadenylated transcription is non- annotated and appears to consist of similar to 3200 missed or alternative exons and 7800 small transcripts of unknown function ( TUFs). Almost half ( 44%) of the detected transcriptional output is non- polyadenylated and probably not protein coding, and of this, 70% overlaps the boundaries of protein- coding genes in a complex manner. Specific analysis of small non- polyadenylated transcripts verified 97% of all annotated small ncRNAs and suggested that the transcriptome contains similar to 1200 small (< 500 nt) unannotated noncoding loci. After combining overlapping transcripts, we estimate that at least 70% of the total C. elegans genome is transcribed.
WOS研究方向	Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Genetics & Heredity
语种	英语
出版者	COLD SPRING HARBOR LAB PRESS, PUBLICATIONS DEPT
WOS记录号	WOS:000249869200008
内容类型	期刊论文
源URL	[http://119.78.100.204/handle/2XEOYT63/11031]
专题	中国科学院计算技术研究所期刊论文_英文
通讯作者	Zhu, Xiaopeng
作者单位	1.Chinese Acad Sci, Inst Biophys, Bioinformat Lab, Beijing 100101, Peoples R China 2.Chinese Acad Sci, Inst Biophys, Natl Lab Biomacromol, Beijing 100101, Peoples R China 3.Chinese Acad Sci, Grad Sch, Beijing 100080, Peoples R China 4.Harvard Univ, Sch Med, Dana Farber Canc Inst, Dept Biostat & Computat Biol, Boston, MA 02115 USA 5.Harvard Univ, Sch Publ Hlth, Boston, MA 02115 USA 6.Affymetrix Inc, Santa Clara, CA 95051 USA 7.Chinese Acad Sci, Inst Comp Technol, Key Lab Intelligent Informat Proc, Bioinformat Res Grp, Beijing 100080, Peoples R China 8.Chinese Natl Human Genome Ctr, Beijing 100176, Peoples R China
推荐引用方式 GB/T 7714	He, Housheng,Wang, Jie,Liu, Tao,et al. Mapping the C-elegans noncoding transcriptome with a whole-genome tiling microarray[J]. GENOME RESEARCH,2007,17(10):1471-1477.
APA	He, Housheng.,Wang, Jie.,Liu, Tao.,Liu, X. Shirley.,Li, Tiantian.,...&Chen, Runsheng.(2007).Mapping the C-elegans noncoding transcriptome with a whole-genome tiling microarray.GENOME RESEARCH,17(10),1471-1477.
MLA	He, Housheng,et al."Mapping the C-elegans noncoding transcriptome with a whole-genome tiling microarray".GENOME RESEARCH 17.10(2007):1471-1477.