Characterization of collision-induced dissociation of deprotonated peptides of 4-16 amino acids using high-resolution mass spectrometry

doi:10.1016/j.ijms.2019.116186

CORC > 计算技术研究所 > 中国科学院计算技术研究所 > 中国科学院计算技术研究所期刊论文 > 英文

	Characterization of collision-induced dissociation of deprotonated peptides of 4-16 amino acids using high-resolution mass spectrometry
	Zuo, Mei-Qing 2,3,4; Sun, Rui-Xiang 3,4; Fang, Run-Qian 1,5; He, Si-Min 5; Dong, Meng-Qiu 3,4
刊名	INTERNATIONAL JOURNAL OF MASS SPECTROMETRY
	2019-11-01
卷号	445 页码:8
关键词	Negative-ion mass spectrometry Peptide fragmentation Collision-induced dissociation (CID) Higher-energy collisional dissociation (HCD) Fragmentation efficiency
ISSN号	1387-3806
DOI	10.1016/j.ijms.2019.116186
英文摘要	Mass spectrometry (MS) analysis of peptides has traditionally been conducted in the positive ion mode on protonated peptides, although negative ion-mode analysis of deprotonated peptides can provide complementary and sometimes critical structural information. This is partly due to insufficient understanding of the fragmentation behaviors of deprotonated peptides. Here, using ion-trap collision-induced dissociation (CID) and higher-energy collisional dissociation (HCD, a beam-type CID), we characterized the fragmentation patterns of 36 deprotonated peptides of 4-16 amino acids on a high-resolution, high-mass accuracy instrument. Our study finds that among the backbone cleavage products, y-, c- and z-type ions (using a nomenclature similar to that of positive ions) are the most dominant species in both CID and HCD spectra of deprotonated peptides, accompanied by abundant neutral loss (NL) peaks. Similar to the charge-dependency of collisional energy of protonated peptides, we find that singly charged deprotonated peptides require significantly higher collisional energy than their doubly or multiply charged counterparts to reach 50% fragmentation. HCD is generally better than CID for peptide sequencing in the negative ion mode, since HCD generates more backbone cleavage products whereas CID produces predominately NL peaks of precursors. For disulfide-bonded peptides and C-terminally amidated peptides, unusual fragmentation is observed in the negative ion mode. The fragmentation behaviors of deprotonated peptides reported in this study will promote further investigation of the fundamental mechanisms and facilitate algorithm development for peptide sequencing in the negative mode. (C) 2019 The Authors. Published by Elsevier B.V.
资助项目	National Natural Science Foundation of China ; Ministry of Science and Technology of China ; Beijing Municipal Science and Technology Commission
WOS研究方向	Physics ; Spectroscopy
语种	英语
出版者	ELSEVIER
WOS记录号	WOS:000489735800001
内容类型	期刊论文
源URL	[http://119.78.100.204/handle/2XEOYT63/4643]
专题	中国科学院计算技术研究所期刊论文_英文
通讯作者	Sun, Rui-Xiang; Dong, Meng-Qiu
作者单位	1.Univ Chinese Acad Sci, Beijing, Peoples R China 2.China Agr Univ, Coll Biol Sci, Beijing, Peoples R China 3.Natl Inst Biol Sci, Beijing, Peoples R China 4.Tsinghua Univ, Tsinghua Inst Multidisciplinary Biomed Res, Beijing, Peoples R China 5.Chinese Acad Sci, Inst Comp Technol, Key Lab Intelligent Informat Proc, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Zuo, Mei-Qing,Sun, Rui-Xiang,Fang, Run-Qian,et al. Characterization of collision-induced dissociation of deprotonated peptides of 4-16 amino acids using high-resolution mass spectrometry[J]. INTERNATIONAL JOURNAL OF MASS SPECTROMETRY,2019,445:8.
APA	Zuo, Mei-Qing,Sun, Rui-Xiang,Fang, Run-Qian,He, Si-Min,&Dong, Meng-Qiu.(2019).Characterization of collision-induced dissociation of deprotonated peptides of 4-16 amino acids using high-resolution mass spectrometry.INTERNATIONAL JOURNAL OF MASS SPECTROMETRY,445,8.
MLA	Zuo, Mei-Qing,et al."Characterization of collision-induced dissociation of deprotonated peptides of 4-16 amino acids using high-resolution mass spectrometry".INTERNATIONAL JOURNAL OF MASS SPECTROMETRY 445(2019):8.