Identification and Functional Demonstration of miRNAs in the Fungus Cryptococcus neoformans

	Identification and Functional Demonstration of miRNAs in the Fungus Cryptococcus neoformans
	Jiang, Nan 1,2; Yang, Yaping 1; Janbon, Guilhem 3; Pan, Jiao 1; Zhu, Xudong 1
刊名	PLOS ONE
	2012-12-26
卷号	7 期号:12 页码:e52734
英文摘要	microRNAs (miRNAs), endogenous posttranscriptional repressors by base-pairing of their cognate mRNAs in plants and animals, have mostly been thought lost in the kingdom of fungi. Here, we report the identification of miRNAs from the fungus Cryptococcus neoformans. With bioinformatics and Northern blotting approaches, we found that these miRNAs and their hairpin precursors were present in this fungus. The size of miR1 and miR2 is 22 nt and 18 nt, respectively. The precursors are about similar to 70 nt in length that is close to mammalian pre-miRNAs. Characteristic features of miRNAs are also found in miR1/2. We demonstrated that the identified miRNAs, miR1 and miR2, caused transgene silencing via the canonical RNAi pathway. Bioinformantics analysis helps to reveal a number of identical sequences of the miR1/2 in transposable elements (TEs) and pseudogenes, prompting us to think that fungal miRNAs might be involved in the regulation of the activity of transposons and the expression of pseudogenes. This study identified functional miRNAs in C. neoformans, and sheds light on the diversity and evolutionary origin of eukaryotic miRNAs.
WOS标题词	Science & Technology
类目[WOS]	Multidisciplinary Sciences
研究领域[WOS]	Science & Technology - Other Topics
关键词[WOS]	TRANSPOSABLE ELEMENTS ; RNA INTERFERENCE ; MICRORNAS ; GENE ; LACCASE ; BIOGENESIS ; MECHANISMS ; EXPRESSION ; VIRULENCE ; PATHWAYS
收录类别	SCI
语种	英语
WOS记录号	WOS:000313618800135
公开日期	2013-01-16
内容类型	期刊论文
源URL	[http://124.16.173.210/handle/312001/306]
专题	天津工业生物技术研究所_基因组分析实验室陈祖耕_期刊论文
作者单位	1.Nankai Univ, Coll Life Sci, Dept Microbiol, Tianjin 300071, Peoples R China 2.Chinese Acad Sci, Tianjin Inst Ind Biotechnol, Tianjin, Peoples R China 3.Inst Pasteur, Dept Mol Mycol, Paris, France
推荐引用方式 GB/T 7714	Jiang, Nan,Yang, Yaping,Janbon, Guilhem,et al. Identification and Functional Demonstration of miRNAs in the Fungus Cryptococcus neoformans[J]. PLOS ONE,2012,7(12):e52734.
APA	Jiang, Nan,Yang, Yaping,Janbon, Guilhem,Pan, Jiao,&Zhu, Xudong.(2012).Identification and Functional Demonstration of miRNAs in the Fungus Cryptococcus neoformans.PLOS ONE,7(12),e52734.
MLA	Jiang, Nan,et al."Identification and Functional Demonstration of miRNAs in the Fungus Cryptococcus neoformans".PLOS ONE 7.12(2012):e52734.