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	RpaA Regulates the Accumulation of Monomeric Photosystem I and PsbA under High Light Conditions in Synechocystis sp PCC 6803
	Majeed, Waqar1; Zhang, Yan2; Xue, Yong1; Ranade, Saurabh1; Blue, Ryan Nastashia1; Wang, Qiang3; He, Qingfang1,2
刊名	PLOS ONE
	2012-09-14
卷号	7期号:9页码:e45139
关键词	SP STRAIN PCC-6803 CYCLIC ADP-RIBOSE PHYSIOLOGICAL SIGNIFICANCE INDUCIBLE POLYPEPTIDES STATE TRANSITIONS GENE-EXPRESSION STOICHIOMETRY CYANOBACTERIA PHOTOPROTECTION PHOTOINHIBITION
ISSN号	1932-6203
通讯作者	Majeed, W (reprint author), Univ Arkansas, Dept Appl Sci, Little Rock, AR 72204 USA.
中文摘要	The response regulator RpaA was examined by targeted mutagenesis under high light conditions in Synechocystis sp. PCC 6803. A significant reduction in chlorophyll fluorescence from photosystem I at 77 K was observed in the RpaA mutant cells under high light conditions. Interestingly, the chlorophyll fluorescence emission from the photosystem I trimers at 77 K are similar to that of the wild type, while the chlorophyll fluorescence from the photosystem I monomers was at a much lower level in the mutant than in the wild type under high light conditions. The RpaA inactivation resulted in a dramatic reduction in the monomeric photosystem I and the D1 protein but not the CP47 content. However, there is no significant difference in the transcript levels of psaA or psbA or other genes examined, most of which are involved in photosynthesis, pigment biosynthesis, or stress responses. Under high light conditions, the growth of the mutant was affected, and both the chlorophyll content and the whole-chain oxygen evolution capability of the mutant were found to be significantly lower than those of the wild type, respectively. We propose that RpaA regulates the accumulation of the monomeric photosystem I and the D1 protein under high light conditions. This is the first report demonstrating that inactivation of a stress response regulator has specifically reduced the monomeric photosystem I. It suggests that PS I monomers and PS I trimers can be regulated independently for acclimation of cells to high light stress.
英文摘要	The response regulator RpaA was examined by targeted mutagenesis under high light conditions in Synechocystis sp. PCC 6803. A significant reduction in chlorophyll fluorescence from photosystem I at 77 K was observed in the RpaA mutant cells under high light conditions. Interestingly, the chlorophyll fluorescence emission from the photosystem I trimers at 77 K are similar to that of the wild type, while the chlorophyll fluorescence from the photosystem I monomers was at a much lower level in the mutant than in the wild type under high light conditions. The RpaA inactivation resulted in a dramatic reduction in the monomeric photosystem I and the D1 protein but not the CP47 content. However, there is no significant difference in the transcript levels of psaA or psbA or other genes examined, most of which are involved in photosynthesis, pigment biosynthesis, or stress responses. Under high light conditions, the growth of the mutant was affected, and both the chlorophyll content and the whole-chain oxygen evolution capability of the mutant were found to be significantly lower than those of the wild type, respectively. We propose that RpaA regulates the accumulation of the monomeric photosystem I and the D1 protein under high light conditions. This is the first report demonstrating that inactivation of a stress response regulator has specifically reduced the monomeric photosystem I. It suggests that PS I monomers and PS I trimers can be regulated independently for acclimation of cells to high light stress.
WOS标题词	Science & Technology
类目[WOS]	Multidisciplinary Sciences
研究领域[WOS]	Science & Technology - Other Topics
关键词[WOS]	SP STRAIN PCC-6803 ; CYCLIC ADP-RIBOSE ; PHYSIOLOGICAL SIGNIFICANCE ; INDUCIBLE POLYPEPTIDES ; STATE TRANSITIONS ; GENE-EXPRESSION ; STOICHIOMETRY ; CYANOBACTERIA ; PHOTOPROTECTION ; PHOTOINHIBITION
收录类别	SCI
资助信息	National Science Foundation [MCB 1120153]; Program on Key Basic Research Project [2011CB200902]
语种	英语
WOS记录号	WOS:000308860100060
公开日期	2013-01-09
内容类型	期刊论文
源URL	[http://ir.ihb.ac.cn/handle/342005/17290]
专题	水生生物研究所_藻类生物学及应用研究中心_期刊论文
作者单位	1.Univ Arkansas, Dept Appl Sci, Little Rock, AR 72204 USA 2.Shandong Acad Agr Sci, High Tech Res Ctr, Jinan, Shandong, Peoples R China 3.Chinese Acad Sci, Inst Hydrobiol, Wuhan, Hubei Province, Peoples R China
推荐引用方式 GB/T 7714	Majeed, Waqar,Zhang, Yan,Xue, Yong,et al. RpaA Regulates the Accumulation of Monomeric Photosystem I and PsbA under High Light Conditions in Synechocystis sp PCC 6803[J]. PLOS ONE,2012,7(9):e45139.
APA	Majeed, Waqar.,Zhang, Yan.,Xue, Yong.,Ranade, Saurabh.,Blue, Ryan Nastashia.,...&He, Qingfang.(2012).RpaA Regulates the Accumulation of Monomeric Photosystem I and PsbA under High Light Conditions in Synechocystis sp PCC 6803.PLOS ONE,7(9),e45139.
MLA	Majeed, Waqar,et al."RpaA Regulates the Accumulation of Monomeric Photosystem I and PsbA under High Light Conditions in Synechocystis sp PCC 6803".PLOS ONE 7.9(2012):e45139.

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