SEPARATION OF THE MINOR FLAVONOLS FROM FLOS GOSSYPII BY HIGH-SPEED COUNTERCURRENT CHROMATOGRAPHY

CORC > 新疆理化技术研究所 > 中国科学院新疆理化技术研究所 > 省部共建新疆特有药用资源利用重点实验室

	SEPARATION OF THE MINOR FLAVONOLS FROM FLOS GOSSYPII BY HIGH-SPEED COUNTERCURRENT CHROMATOGRAPHY
	Yang Yi; Zhao Yongxin; Gu Dongyu; Ayupbek Amatjan; Huang Yun; Dou Jun; Ito Yoichiro; Zhang Tianyou; Aisa Haji Akber
刊名	JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES
	2010
卷号	33 期号:16 页码:1502-1515
关键词	flavonol Flos gossypii HSCCC separation rules of flavonols
ISSN号	10826076
英文摘要	An effective high-speed countercurrent chromatography (HSCCC) method was established for further separation and purification of four minor flavonols in addition to five major flavonols which were reported by our previous study from extracts of Flos gossypii. HSCCC was performed with three two-phase solvent systems composed of n-hexane-ethyl acetate-methanol-water (7.5:15:6:7, v/v), (2.5:15:2:7, v/v), and (0:1:0:1, v/v). The separation was repeated three times, and 3.8mg of 8-methoxyl-kaempferol-7-O-β-D-rhamnoside (HPLC purity 98.27%), 6.7mg of astragalin (HPLC purity 94.18%), 3.3mg of 4'-methoxyl-quercetin-7-O- β-D-glucoside (HPLC purity 94.30%), and 8.2mg of hyperoside (HPLC purity 93.48%) were separated from 150mg of the crude sample. The chemical structures of the flavonols were confirmed by MS, 1H NMR, and 13C NMR. Meanwhile, the results indicated that the target compound with smaller K value (<0.5) can be separated by increasing column length of HSCCC. And, four separation rules of flavonols were established according to the present study and references were summarized, which can be used as a useful guide for separation of flavonols by HSCCC.
学科主题	Biochemistry & Molecular Biology ; Chemistry
收录类别	SCI
WOS记录号	WOS:000282581800002
公开日期	2012-11-29
内容类型	期刊论文
源URL	[http://ir.xjipc.cas.cn/handle/365002/1786]
专题	新疆理化技术研究所_省部共建新疆特有药用资源利用重点实验室新疆理化技术研究所_资源化学研究室
作者单位	Biochem & Biophys Ctr, Bioseparat Technol Lab, NHLBI, NIH, Bethesda, MD 20892 USA;Chinese Acad Sci, Xinjiang Key Lab Plant Resources & Nat Prod Chem, Xinjiang Tech Inst Phys & Chem, Urumqi, Peoples R China;Chinese Acad Sci, Key Lab Chem Plant Resources Arid Area, Xinjiang Tech Inst Phys & Chem, Urumqi, Peoples R China;Beijing Inst New Technol Applicat, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Yang Yi,Zhao Yongxin,Gu Dongyu,et al. SEPARATION OF THE MINOR FLAVONOLS FROM FLOS GOSSYPII BY HIGH-SPEED COUNTERCURRENT CHROMATOGRAPHY[J]. JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES,2010,33(16):1502-1515.
APA	Yang Yi.,Zhao Yongxin.,Gu Dongyu.,Ayupbek Amatjan.,Huang Yun.,...&Aisa Haji Akber.(2010).SEPARATION OF THE MINOR FLAVONOLS FROM FLOS GOSSYPII BY HIGH-SPEED COUNTERCURRENT CHROMATOGRAPHY.JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES,33(16),1502-1515.
MLA	Yang Yi,et al."SEPARATION OF THE MINOR FLAVONOLS FROM FLOS GOSSYPII BY HIGH-SPEED COUNTERCURRENT CHROMATOGRAPHY".JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES 33.16(2010):1502-1515.