Evaluation of the performance of protein separation in figure-8 centrifugal counter-current chromatography

CORC > 新疆理化技术研究所 > 中国科学院新疆理化技术研究所 > 省部共建新疆特有药用资源利用重点实验室

	Evaluation of the performance of protein separation in figure-8 centrifugal counter-current chromatography
	Yang Yi; Gu Dongyu; Aisa Haji Akber; Ito Yoichiro
刊名	JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
	2011
卷号	879 期号:32 页码:3802-3805
关键词	Centrifugal counter-current chromatography Figure-8 column Protein separation Polymer phase system Lysozyme Myoglobin Hollow fiber membrane dialyzer
ISSN号	15700232
英文摘要	The performance of protein separation using the figure-8 column configuration in centrifugal countercurrent chromatography was investigated under various flow rates and revolution speeds. The separation was performed with a two-phase solvent system composed of polyethylene glycol 1000/potassium phosphate each at 12.5% (w/w) in water and with lysozyme and myoglobin as test samples. In order to improve tracing of the elution curve, a hollow fiber membrane dialyzer was inserted at the inlet of the UV detector. The results showed that the retention of stationary phase (Sf) and resolution (Rs) increased with decreased flow rate and increased revolution speed. The highest Rs of approximately 1 was obtained at a flow rate of 0.01 mL/min under a revolution speed of 1200 rpm with a 3.4 mL capacity column.
学科主题	Biochemistry & Molecular Biology ; Chemistry
WOS记录号	WOS:000298530200004
公开日期	2012-11-29
内容类型	期刊论文
源URL	[http://ir.xjipc.cas.cn/handle/365002/1449]
专题	新疆理化技术研究所_省部共建新疆特有药用资源利用重点实验室
作者单位	NHLBI, Bioseparat Technol Lab, Biochem & Biophys Ctr, NIH, Bethesda, MD 20892 USA;Chinese Acad Sci, Key Lab Chem Plant Resources Arid Reg, Xinjiang Tech Inst Phys & Chem, Urumqi 830011, Peoples R China
推荐引用方式 GB/T 7714	Yang Yi,Gu Dongyu,Aisa Haji Akber,et al. Evaluation of the performance of protein separation in figure-8 centrifugal counter-current chromatography[J]. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,2011,879(32):3802-3805.
APA	Yang Yi,Gu Dongyu,Aisa Haji Akber,&Ito Yoichiro.(2011).Evaluation of the performance of protein separation in figure-8 centrifugal counter-current chromatography.JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,879(32),3802-3805.
MLA	Yang Yi,et al."Evaluation of the performance of protein separation in figure-8 centrifugal counter-current chromatography".JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES 879.32(2011):3802-3805.