studies on the performance of different coiled column configurations for compact type-i countercurrent chromatography

CORC > 新疆理化技术研究所 > 中国科学院新疆理化技术研究所 > 省部共建新疆特有药用资源利用重点实验室

	studies on the performance of different coiled column configurations for compact type-i countercurrent chromatography
	Yang Yi; Gu Dongyu; Aisa Haji Akber; Ito Yoichiro
刊名	Journal of Separation Science
	2011
卷号	34 期号:10 页码:1205-1211
关键词	Coiled column configuration Compact type-I countercurrent chromatography Dinitrophenyl amino acid Dipeptide Retention of the stationary phase Resolution
ISSN号	16159306
英文摘要	Three types of novel coiled column configurations, i.e. a triangular coiled column and elliptical coiled columns I and II, were designed for type-I countercurrent chromatography and their performances were evaluated with two solvent systems each with suitable test samples. Three dinitrophenyl (DNP) amino acids (DNP-DL-glu, DNP-β-ala and DNP-L-ala) were separated with a moderately hydrophobic two-phase solvent system composed of hexane-ethyl acetate-metanol-0.1 M hydrochloric acid (1:1:1:1, v/v), while two dipeptides (tryptophyl-tyrosine and valyl-tyrosine) were separated with a polar solvent system composed of 1-butanol-acetic acid-water (4.75:0.25:5, v/v). The overall results indicated that the performance of compact type-I countercurrent chromatography was improved by elliptical coiled column I which was mounted with its maximum coil diameter perpendicular to the surface of the column holder. Hydrodynamic effects involved in these separations were discussed. Copyright © 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
学科主题	Chemistry
WOS记录号	WOS:000290635400016
公开日期	2012-11-29
内容类型	期刊论文
源URL	[http://ir.xjipc.cas.cn/handle/365002/1446]
专题	新疆理化技术研究所_省部共建新疆特有药用资源利用重点实验室
作者单位	NHLBI, Bioseparat Technol Lab, Biochem & Biophys Ctr, NIH, Bethesda, MD 20892 USA;Chinese Acad Sci, Key Lab Xinjiang Indigenous Med Plants Resource U, Xinjiang Tech Inst Phys & Chem, Urumqi, Peoples R China
推荐引用方式 GB/T 7714	Yang Yi,Gu Dongyu,Aisa Haji Akber,et al. studies on the performance of different coiled column configurations for compact type-i countercurrent chromatography[J]. Journal of Separation Science,2011,34(10):1205-1211.
APA	Yang Yi,Gu Dongyu,Aisa Haji Akber,&Ito Yoichiro.(2011).studies on the performance of different coiled column configurations for compact type-i countercurrent chromatography.Journal of Separation Science,34(10),1205-1211.
MLA	Yang Yi,et al."studies on the performance of different coiled column configurations for compact type-i countercurrent chromatography".Journal of Separation Science 34.10(2011):1205-1211.