Preparation and characterization of enrofloxacin-imprinted monolith prepared with crowding agents

CORC > 新疆理化技术研究所 > 中国科学院新疆理化技术研究所 > 省部共建新疆特有药用资源利用重点实验室

	Preparation and characterization of enrofloxacin-imprinted monolith prepared with crowding agents
	Li Xiao-Xia; Bai Li-Hong; Wang Huan; Wang Jia; Huang Yan-Ping; Liu Zhao-Sheng
刊名	Journal of Chromatography A
	2012
卷号	1251 期号:6 页码:141-147
关键词	Monolith Molecularly imprinted polymer Molecular crowding Molecular recognition Enrofloxacin
ISSN号	219673
英文摘要	Molecular crowding is a new approach to promoting molecular imprinting more efficiently. In this work, this concept was applied to the preparation of imprinted monoliths in the presence of molecular crowding agent for stabilizing binding sites and improving molecular recognition. The imprinted monolithic column was synthesized using a mixture of enrofloxacin (template), methacrylic acid, ethylene glycol dimethacrylate, and polystyrene (molecular crowding agent). Some polymerization factors, such as template-monomer molar ratio and the composition of the porogen, on the imprinting effect of resulting MIP monolith were systematically investigated. Moreover, a stoichiometric displacement model for retention was successfully applied to evaluate the interaction between the solute and the stationary phase. Compared with the MIP prepared by conventional polymerization, the molecular crowding-based monolithic MIPs showed higher selectivity. The results suggested that molecular crowding is a powerful strategy to increase the effect of molecular imprinting.
学科主题	Biochemistry & Molecular Biology ; Chemistry
WOS记录号	WOS:000307428800017
公开日期	2012-11-29
内容类型	期刊论文
源URL	[http://ir.xjipc.cas.cn/handle/365002/1397]
专题	新疆理化技术研究所_省部共建新疆特有药用资源利用重点实验室
作者单位	Chinese Acad Sci, Xinjiang Tech Inst Phys & Chem, Key Lab Plant Resources & Chem Arid Zone, Urumqi 830011, Xinjiang, Peoples R China;Tianjin Med Univ, Sch Pharm, Tianjin Key Lab Technol Enabling Dev Clin Therape, Tianjin 300070, Peoples R China
推荐引用方式 GB/T 7714	Li Xiao-Xia,Bai Li-Hong,Wang Huan,et al. Preparation and characterization of enrofloxacin-imprinted monolith prepared with crowding agents[J]. Journal of Chromatography A,2012,1251(6):141-147.
APA	Li Xiao-Xia,Bai Li-Hong,Wang Huan,Wang Jia,Huang Yan-Ping,&Liu Zhao-Sheng.(2012).Preparation and characterization of enrofloxacin-imprinted monolith prepared with crowding agents.Journal of Chromatography A,1251(6),141-147.
MLA	Li Xiao-Xia,et al."Preparation and characterization of enrofloxacin-imprinted monolith prepared with crowding agents".Journal of Chromatography A 1251.6(2012):141-147.