Separation and Purification of Phenolic Acids and Myricetin from Black Currant by High-Speed Countercurrent Chromatography

CORC > 新疆理化技术研究所 > 中国科学院新疆理化技术研究所 > 省部共建新疆特有药用资源利用重点实验室

	Separation and Purification of Phenolic Acids and Myricetin from Black Currant by High-Speed Countercurrent Chromatography
	He Dajun; Gu Dongyu; Huang Yun; Ayupbek Amatjan; Yang Yi; Aisa Haji Akber; Ito Yoichiro
刊名	JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES
	2009
卷号	32 期号:20 页码:3077-3088
关键词	Black currant Caffeic acid High-speed countercurrent chromatography (HSCCC) 4-Hydroxybenzoic acid Myricetin Protocatechuic acid
ISSN号	10826076
英文摘要	Black currant is an important material in the food industry, but little research has been reported on the isolation of phenolic acids because of their low content. In the present study, high-speed countercurrent chromatography (HSCCC) has been successfully used for the preparative isolation of the minor phenolic compounds from the ethyl acetate extracts of black currant fruit. The HSCCC separation was performed with a two phase solvent system composed of n-hexane/EtOAc/MeOH/H2O (5:15:4:7v/v) at a flow rate of 1.5mL/min. From a 500mg crude sample 0.8mg of protocatechuic acid, 1.0mg of caffeic acid, 0.5mg of 4-hydroxybenzoic acid, and 2.5mg of myricetin were purified by a one step HSCCC operation,. Their chemical structures were confirmed by MS and NMR.
学科主题	Biochemistry & Molecular Biology ; Chemistry
WOS记录号	WOS:000271822900013
公开日期	2012-11-29
内容类型	期刊论文
源URL	[http://ir.xjipc.cas.cn/handle/365002/1848]
专题	新疆理化技术研究所_省部共建新疆特有药用资源利用重点实验室新疆理化技术研究所_资源化学研究室
作者单位	NHLBI, Bioseparat Technol Lab, Biochem & Biophys Ctr, NIH, Bethesda, MD 20892 USA;Chinese Acad Sci, Xinjiang Tech Inst Phys & Chem, Xinjiang Key Lab Plant Resources & Nat Prod Chem, Urumqi 830011, Peoples R China;Union Labs Bioact Ingredients Edible Plants Cent, Urumqi, Peoples R China;Shihezi Univ, Sch Life Sci, Shihezi, Peoples R China;Chinese Acad Sci, Grad Univ, Beijing, Peoples R China
推荐引用方式 GB/T 7714	He Dajun,Gu Dongyu,Huang Yun,et al. Separation and Purification of Phenolic Acids and Myricetin from Black Currant by High-Speed Countercurrent Chromatography[J]. JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES,2009,32(20):3077-3088.
APA	He Dajun.,Gu Dongyu.,Huang Yun.,Ayupbek Amatjan.,Yang Yi.,...&Ito Yoichiro.(2009).Separation and Purification of Phenolic Acids and Myricetin from Black Currant by High-Speed Countercurrent Chromatography.JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES,32(20),3077-3088.
MLA	He Dajun,et al."Separation and Purification of Phenolic Acids and Myricetin from Black Currant by High-Speed Countercurrent Chromatography".JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES 32.20(2009):3077-3088.