Simultaneous separation and purification of flavonoids and oleuropein from Olea europaea L. (olive) leaves usingmacroporous resin

CORC > 兰州化学物理研究所 > 中国科学院兰州化学物理研究所 > 中科院西北特色植物资源化学重点实验室/甘肃省天然药物重点实验室

	Simultaneous separation and purification of flavonoids and oleuropein from Olea europaea L. (olive) leaves usingmacroporous resin
	Di DL(邸多隆); Di DL(邸多隆)
刊名	Journal of the Science of Food and Agriculture
	2011
卷号	91 页码:2826-2834
关键词	macroporous resins flavonoids oleuropein Olea europaea L. leaves purification
ISSN号	0022-5142
通讯作者	邸多隆
中文摘要	BACKGROUND:This study developed a feasible process tosimultaneously separateandpurifypolyphenols, including flavonoids and oleuropein, from the leaves of Olea europaea L. Macroporous resins were used as the separation and purificationmaterials. The performance and separation capabilities of eight resins (D101, DM130, HPD450, LSA-21, LSA-40, 07C, LSD001 and HPD600) were systematically evaluated. The contents of target polyphenols in different extracts were determined using ultraviolet (for flavonoids) and high-performance liquid chromatographic (for oleuropein) methods. The static adsorption and desorption results showed that resin LSA-21 had better adsorption properties among the eight resins. Influential factors such as extraction method, pH value of feeding solution, desorption solution, adsorption kinetics and adsorption isotherm, etc. to the extraction and purification of these polyphenols were successively investigated on resin LSA-21. RESULTS: The target flavonoids and oleuropein were selectively purified using resin LSA-21. Compared with the contents in raw leaves, the contents of total flavonoids and oleuropein in the final purified products were increased 13.2-fold (from 16 to 211 g kg−1) and 7.5-fold (from 120 to 902 g kg−1) with recovery yields of 87.9% and 85.6%, respectively. CONCLUSION: This extraction and purificationmethod could be used in the large-scale enrichment or purification of flavonoids, oleuropein and other polyphenols from O. europaea L. leaves or other herbal materials in industrial, food processing and medicalmanufacture.
学科主题	分析化学与药物化学
收录类别	SCI
资助信息	the “Hundred Talents Program” of the Chinese Academy of Sciences (CAS);the National Natural Sciences Foundation of China (NSFC No. 20974116);the project of “Scientific and Technical Support to Gansu Province” of CAS (KJZG-2009-02)
语种	英语
公开日期	2012-10-18
内容类型	期刊论文
源URL	[http://210.77.64.217/handle/362003/1556]
专题	兰州化学物理研究所_中科院西北特色植物资源化学重点实验室/甘肃省天然药物重点实验室
通讯作者	Di DL(邸多隆); Di DL(邸多隆)
推荐引用方式 GB/T 7714	Di DL,Di DL. Simultaneous separation and purification of flavonoids and oleuropein from Olea europaea L. (olive) leaves usingmacroporous resin[J]. Journal of the Science of Food and Agriculture,2011,91:2826-2834.
APA	邸多隆,&邸多隆.(2011).Simultaneous separation and purification of flavonoids and oleuropein from Olea europaea L. (olive) leaves usingmacroporous resin.Journal of the Science of Food and Agriculture,91,2826-2834.
MLA	邸多隆,et al."Simultaneous separation and purification of flavonoids and oleuropein from Olea europaea L. (olive) leaves usingmacroporous resin".Journal of the Science of Food and Agriculture 91(2011):2826-2834.