Ultra-performance LC-photodiode array-el-ESI-MS/MS screening method for the detection of radical-scavenging natural antioxidants from Radix et Rhizoma Rhei

CORC > 兰州化学物理研究所 > 中国科学院兰州化学物理研究所 > 中科院西北特色植物资源化学重点实验室/甘肃省天然药物重点实验室

	Ultra-performance LC-photodiode array-el-ESI-MS/MS screening method for the detection of radical-scavenging natural antioxidants from Radix et Rhizoma Rhei
	Shi YP(师彦平); Shi YP(师彦平)
刊名	Journal of Separation Science
	2011
卷号	34 页码:268-277
关键词	Antioxidants 1-O-Galloyl-2-O-cinnamoylglucose Radical-scavenging Radix et Rhizoma Rhei Ultra-performance LC-PDA-el-ESI-MS/MS
ISSN号	1615-9306
通讯作者	师彦平
中文摘要	A novel ultra-performance LC-photodiode array-el-ESI-MS/MS screening method was developed for the detection and identification of natural antioxidants from Radix et Rhizoma Rhei. Nine compounds were found to possess a potential antioxidant activity, and their free radical-scavenging capacities were investigated in detail. The nine compounds were identified as 1-O-galloyl-2-O-cinnamoylglucose, 6-hydroxymusizin-8-O-β-D-glucopyranoside,(1)-catechin, gallic acid 3-O-β-D-glucopyranoside, trans-3,5,40-trihydroxystilbene-40-O-β-D-(200-O-galloyl)-glucopyranoside, sennoside A, 4-(40-hydroxyphenyl)-2-butanone-40-O-β-D-(200-O-galloyl-600-O-p-coumaroyl) glucopyranoside, emodin-8-O-(60-Omalonyl) glucopyranoside, and physcion-8-O-β-D-glucopyranoside. The reactivity and SC50 values of those compounds were investigated, respectively. 1-O-Galloyl-2-O-cinnamoylglucose showed the strongest capability for scavenging 1,1-diphenyl-2-picrylhydrazylfree radical; trans-3,5,40-trihydroxystilbene-40-O-β-D-(200-O-galloyl) glucopyranoside showed the strongest capability for scavenging superoxide radical; 4-(40-hydroxyphenyl)-2-butanone-40-O-2-D-(200-O-galloyl-600-O-p-coumaroyl) glucopyranoside exhibited the highest reactivity in the lipid peroxidation processes. The use of the analytical screening method based on ultra-performance LC-photodiode array-el-ESI-MS/MS would provide a new way for rapid detection of radical-scavenging natural compounds from Radix et Rhizoma Rhei or complex matrices.
学科主题	分析化学与药物化学
收录类别	SCI
资助信息	the National Key Technology Research and Development Program of China (No. 2007BAI37B05);the Western high-tech Project Action Plan of Chinese Academy of Sciences (No. KG-CX2-YW-510)
语种	英语
公开日期	2012-10-18
内容类型	期刊论文
源URL	[http://210.77.64.217/handle/362003/1519]
专题	兰州化学物理研究所_中科院西北特色植物资源化学重点实验室/甘肃省天然药物重点实验室
通讯作者	Shi YP(师彦平); Shi YP(师彦平)
推荐引用方式 GB/T 7714	Shi YP,Shi YP. Ultra-performance LC-photodiode array-el-ESI-MS/MS screening method for the detection of radical-scavenging natural antioxidants from Radix et Rhizoma Rhei[J]. Journal of Separation Science,2011,34:268-277.
APA	师彦平,&师彦平.(2011).Ultra-performance LC-photodiode array-el-ESI-MS/MS screening method for the detection of radical-scavenging natural antioxidants from Radix et Rhizoma Rhei.Journal of Separation Science,34,268-277.
MLA	师彦平,et al."Ultra-performance LC-photodiode array-el-ESI-MS/MS screening method for the detection of radical-scavenging natural antioxidants from Radix et Rhizoma Rhei".Journal of Separation Science 34(2011):268-277.