Metagenomic Insights into the Fibrolytic Microbiome in Yak Rumen

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	Metagenomic Insights into the Fibrolytic Microbiome in Yak Rumen
	Dai, Xin 1; Zhu, Yaxin 1; Luo, Yingfeng 2; Song, Lei 1; Liu, Di 3; Liu, Li 3; Chen, Furong 1; Wang, Min 1; Li, Jiabao 1; Zeng, Xiaowei 4
刊名	PLOS ONE
	2012-07-13
卷号	7 期号:7 页码:e40430
英文摘要	The rumen hosts one of the most efficient microbial systems for degrading plant cell walls, yet the predominant cellulolytic proteins and fibrolytic mechanism(s) remain elusive. Here we investigated the cellulolytic microbiome of the yak rumen by using a combination of metagenome-based and bacterial artificial chromosome (BAC)-based functional screening approaches. Totally 223 fibrolytic BAC clones were pyrosequenced and 10,070 ORFs were identified. Among them 150 were annotated as the glycoside hydrolase (GH) genes for fibrolytic proteins, and the majority (69%) of them were clustered or linked with genes encoding related functions. Among the 35 fibrolytic contigs of >10 Kb in length, 25 were derived from Bacteroidetes and four from Firmicutes. Coverage analysis indicated that the fibrolytic genes on most Bacteroidetes-contigs were abundantly represented in the metagenomic sequences, and they were frequently linked with genes encoding SusC/SusD-type outer-membrane proteins. GH5, GH9, and GH10 cellulase/hemicellulase genes were predominant, but no GH48 exocellulase gene was found. Most (85%) of the cellulase and hemicellulase proteins possessed a signal peptide; only a few carried carbohydrate-binding modules, and no cellulosomal domains were detected. These findings suggest that the SucC/SucD-involving mechanism, instead of one based on cellulosomes or the free-enzyme system, serves a major role in lignocellulose degradation in yak rumen. Genes encoding an endoglucanase of a novel GH5 subfamily occurred frequently in the metagenome, and the recombinant proteins encoded by the genes displayed moderate Avicelase in addition to endoglucanase activities, suggesting their important contribution to lignocellulose degradation in the exocellulase-scarce rumen.
学科主题	功能基因组
WOS标题词	Science & Technology
类目[WOS]	Multidisciplinary Sciences
研究领域[WOS]	Science & Technology - Other Topics
关键词[WOS]	OUTER-MEMBRANE PROTEINS ; BACTEROIDES-THETAIOTAOMICRON ; GLYCOSIDE HYDROLASES ; BACTERIAL ; GENOME ; GENES ; DEGRADATION ; DIVERSITY ; DISCOVERY ; CELLULOSE
收录类别	SCI
语种	英语
WOS记录号	WOS:000306406700028
公开日期	2012-11-23
内容类型	期刊论文
源URL	[http://ir.qibebt.ac.cn:8080/handle/337004/1423]
专题	青岛生物能源与过程研究所_单细胞中心
作者单位	1.Chinese Acad Sci, Inst Microbiol, State Key Lab Microbial Resources, Beijing, Peoples R China 2.Chinese Acad Sci, Beijing Inst Gen, CAS Key Lab Genome Sci & Informat, Beijing, Peoples R China 3.Chinese Acad Sci, Inst Microbiol, Bioinformat Ctr, Beijing, Peoples R China 4.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioprocess Technol, Qingdao, Peoples R China
推荐引用方式 GB/T 7714	Dai, Xin,Zhu, Yaxin,Luo, Yingfeng,et al. Metagenomic Insights into the Fibrolytic Microbiome in Yak Rumen[J]. PLOS ONE,2012,7(7):e40430.
APA	Dai, Xin.,Zhu, Yaxin.,Luo, Yingfeng.,Song, Lei.,Liu, Di.,...&Dong, Xiuzhu.(2012).Metagenomic Insights into the Fibrolytic Microbiome in Yak Rumen.PLOS ONE,7(7),e40430.
MLA	Dai, Xin,et al."Metagenomic Insights into the Fibrolytic Microbiome in Yak Rumen".PLOS ONE 7.7(2012):e40430.