Calcium influx through hyperpolarization-activated cation channels (I-h channels) contributes to activity-evoked neuronal secretion

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	Calcium influx through hyperpolarization-activated cation channels (I-h channels) contributes to activity-evoked neuronal secretion
	Yu, X ; Duan, KL ; Shang, CF ; Yu, HG ; Zhou, Z
刊名	PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
	2004
卷号	101 期号:4 页码:1051-1056
关键词	ADRENAL CHROMAFFIN CELLS NUCLEOTIDE-GATED CHANNELS LONG-TERM POTENTIATION RECEPTOR CHANNELS PACEMAKER CHANNELS SYNAPTIC-TRANSMISSION VENTRICULAR MYOCYTES FACILITATION PERMEATION EXPRESSION
ISSN号	0027-8424
通讯作者	Zhou, Z (reprint author), Chinese Acad Sci, Inst Neurosci, Shanghai Inst Biol Sci, 320 Yue Yang Rd, Shanghai 200031, Peoples R China,zzhou@ion.ac.cn
英文摘要	The hyperpolarization-activated cation channels (I-h) play a distinct role in rhythmic activities in a variety of tissues, including neurons and cardiac cells. in the present study, we investigated whether Ca2+ can permeate through the hyperpolarization-activated pacemaker channels (HCN) expressed in HEK293 cells and I-h channels in dorsal root ganglion (DRG) neurons. Using combined measurements of whole-cell currents and fura-2 Ca2+ imaging, we found that there is a Ca2(+) influx in proportion to I-h induced by hyperpolarization in HEK293 cells. The I-h channel blockers Cs+ and ZD7288 inhibit both HCN current and Ca2(+) influx. Measurements of the fractional Ca2+ current showed that it constitutes 0.60+/-0.02% of the net inward current through HCN4 at -120 mV. This fractional current is similar to that of the low Ca2+-permeable AMPA-R (alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptor) channels in Purkinje neurons. In DRG neurons, activation of I-h for 30 s also resulted in a Ca2+ influx and an elevated action potential-induced secretion, as assayed by the increase in membrane capacitance. These results suggest a functional significance for I-h channels in modulating neuronal secretion by permitting Ca2+ influx at negative membrane potentials.
学科主题	Science & Technology - Other Topics
收录类别	SCI
语种	英语
公开日期	2012-07-23
内容类型	期刊论文
源URL	[http://ir.sibs.ac.cn/handle/331001/1978]
专题	上海神经科学研究所_神经所(总)
推荐引用方式 GB/T 7714	Yu, X,Duan, KL,Shang, CF,et al. Calcium influx through hyperpolarization-activated cation channels (I-h channels) contributes to activity-evoked neuronal secretion[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,2004,101(4):1051-1056.
APA	Yu, X,Duan, KL,Shang, CF,Yu, HG,&Zhou, Z.(2004).Calcium influx through hyperpolarization-activated cation channels (I-h channels) contributes to activity-evoked neuronal secretion.PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,101(4),1051-1056.
MLA	Yu, X,et al."Calcium influx through hyperpolarization-activated cation channels (I-h channels) contributes to activity-evoked neuronal secretion".PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 101.4(2004):1051-1056.