Calcium- and dynamin-independent endocytosis in dorsal root ganglion neurons

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	Calcium- and dynamin-independent endocytosis in dorsal root ganglion neurons
	Zhang, C ; Xiong, W ; Zheng, H ; Wang, LC ; Lu, B ; Zhou, Z
刊名	NEURON
	2004
卷号	42 期号:2 页码:225-236
关键词	ADRENAL CHROMAFFIN CELLS TEMPERATURE-SENSITIVE MUTANT SYNAPTIC VESICLE ENDOCYTOSIS TRANSMITTER RELEASE RECEPTOR INTERNALIZATION DROSOPHILA-MELANOGASTER HIPPOCAMPAL SYNAPSES REVERSIBLE BLOCKAGE NEUROTROPHIC FACTOR MEMBRANE RETRIEVAL
ISSN号	0896-6273
通讯作者	Zhou, Z (reprint author), Peking Univ, Inst Mol Med, Beijing 100871, Peoples R China,zzhou@ion.ac.cn
英文摘要	Synaptic vesicle endocytosis is believed to require calcium and the GTPase dynamin. We now report a form of rapid endocytosis (RE) in dorsal root ganglion (DRG) neurons that, unlike previously described forms of endocytosis, is independent of calcium and dynamin. The RE is tightly coupled to calcium-independent but voltage-dependent secretion (CIVDS). Using FM dye and capacitance measurements, we show that membrane depolarization induces RE in the absence of calcium. Inhibition of dynamin function does not affect RE. The magnitude of RE is proportional to that of preceding CIVDS and stimulation frequency. Inhibitors of protein kinase A (PKA) suppress RE induced by high-frequency depolarization, while PKA activators enhance RE induced by low-frequency depolarization. Biochemical experiments demonstrate that depolarization directly upregulates PKA activity in calcium-free medium. These results reveal a calcium- and dynamin-independent form of endocytosis, which is controlled by neuronal activity and PKA-dependent phosphorylation, in DRG neurons.
学科主题	Neurosciences & Neurology
收录类别	SCI
语种	英语
公开日期	2012-07-23
内容类型	期刊论文
源URL	[http://ir.sibs.ac.cn/handle/331001/1966]
专题	上海神经科学研究所_神经所(总)
推荐引用方式 GB/T 7714	Zhang, C,Xiong, W,Zheng, H,et al. Calcium- and dynamin-independent endocytosis in dorsal root ganglion neurons[J]. NEURON,2004,42(2):225-236.
APA	Zhang, C,Xiong, W,Zheng, H,Wang, LC,Lu, B,&Zhou, Z.(2004).Calcium- and dynamin-independent endocytosis in dorsal root ganglion neurons.NEURON,42(2),225-236.
MLA	Zhang, C,et al."Calcium- and dynamin-independent endocytosis in dorsal root ganglion neurons".NEURON 42.2(2004):225-236.