Essential role of TRPC channels in the guidance of nerve growth cones by brain-derived neurotrophic factor

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	Essential role of TRPC channels in the guidance of nerve growth cones by brain-derived neurotrophic factor
	Li, Y ; Jia, YC ; Cui, K ; Li, N ; Zheng, ZY ; Wang, YZ ; Yuan, XB
刊名	NATURE
	2005
卷号	434 期号:7035 页码:894-898
关键词	PHOSPHOLIPASE-C CATION CHANNELS AXON GUIDANCE CELLS RECEPTORS ACTIVATION INHIBITOR CURRENTS FAMILY BDNF
ISSN号	0028-0836
通讯作者	Yuan, XB (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Neurosci, Shanghai 200031, Peoples R China,yzwang@ion.ac.cn ; yuanxb@ion.ac.cn
英文摘要	Brain-derived neurotrophic factor ( BDNF) is known to promote neuronal survival and differentiation(1) and to guide axon extension both in vitro(2,3) and in vivo(4). The BDNF-induced chemoattraction of axonal growth cones requires Ca2+ signalling(3), but how Ca2+ is regulated by BDNF at the growth cone remains largely unclear. Extracellular application of BDNF triggers membrane currents resembling those through TRPC ( transient receptor potential canonical) channels in rat pontine neurons(5) and in Xenopus spinal neurons(6). Here, we report that in cultured cerebellar granule cells, TRPC channels contribute to the BDNF-induced elevation of Ca2+ at the growth cone and are required for BDNF-induced chemo-attractive turning. Several members of the TRPC family are highly expressed in these neurons, and both Ca2+ elevation and growth-cone turning induced by BDNF are abolished by pharmacological inhibition of TRPC channels, overexpression of a dominant-negative form of TRPC3 or TRPC6, or downregulation of TRPC3 expression via short interfering RNA. Thus, TRPC channel activity is essential for nerve-growth-cone guidance by BDNF.
学科主题	Science & Technology - Other Topics
收录类别	SCI
语种	英语
公开日期	2012-07-23
内容类型	期刊论文
源URL	[http://ir.sibs.ac.cn/handle/331001/1911]
专题	上海神经科学研究所_神经所(总)
推荐引用方式 GB/T 7714	Li, Y,Jia, YC,Cui, K,et al. Essential role of TRPC channels in the guidance of nerve growth cones by brain-derived neurotrophic factor[J]. NATURE,2005,434(7035):894-898.
APA	Li, Y.,Jia, YC.,Cui, K.,Li, N.,Zheng, ZY.,...&Yuan, XB.(2005).Essential role of TRPC channels in the guidance of nerve growth cones by brain-derived neurotrophic factor.NATURE,434(7035),894-898.
MLA	Li, Y,et al."Essential role of TRPC channels in the guidance of nerve growth cones by brain-derived neurotrophic factor".NATURE 434.7035(2005):894-898.