The timing of endocytosis after activation of a G-protein-coupled receptor in a sensory neuron

CORC > 上海神经科学研究所 > 中国科学院上海生命科学研究院神经科学研究所 > 神经所(总)

	The timing of endocytosis after activation of a G-protein-coupled receptor in a sensory neuron
	Wang, LC ; Xiong, W ; Zheng, J ; Zhou, Y ; Zheng, H ; Zhang, C ; Zheng, LH ; Zhu, XL ; Xiong, ZQ ; Wang, LY ; Cheng, HP ; Zhou, Z(周专)
刊名	BIOPHYSICAL JOURNAL
	2006
卷号	90 期号:10 页码:3590-3598
关键词	ROOT GANGLION NEURONS ADRENAL CHROMAFFIN CELLS CLATHRIN-MEDIATED ENDOCYTOSIS FROG NEUROMUSCULAR JUNCTION TRANSMITTER RELEASE RAPID ENDOCYTOSIS NERVE-TERMINALS CENTRAL SYNAPSE CALCIUM INFLUX RAT ADIPOCYTES
ISSN号	0006-3495
通讯作者	Zhou, Z (reprint author), Peking Univ, Inst Mol Med, 5 Yiheyuan Rd, Beijing 100871, Peoples R China,zzhou@pku.edu.cn
英文摘要	Endocytosis is a fundamental cellular event in membrane retrieval after exocytosis and in the regulation of receptor-mediated signal transduction. In contrast to the well-studied depolarization-induced membrane recycling, little is known about the kinetics of ligand-induced endocytosis of G-protein-coupled receptors in neurons. Here we investigated the kinetics of ligand-receptor binding-induced endocytosis in rat sensory neurons using a membrane capacitance assay. The time constant of ADP-induced endocytosis of P2Y-receptors was determined as 1.7 s. The ADP-induced endocytosis was blocked by antagonists against P2Y, phosphorylation, and clathrin. However, block of dynamin was without effect. The ADP-induced endocytosis was confirmed independently by a single vesicle image technique using a styryl FM2-10. Finally, the receptors were internalized in response to ADP, as determined by GFP-labeled P2Y. We conclude that ligand-receptor binding leads to rapid endocytosis in the cytoplasm of rat dorsal root ganglion neurons.
学科主题	Biophysics
收录类别	SCI
语种	英语
公开日期	2012-07-23
内容类型	期刊论文
源URL	[http://ir.sibs.ac.cn/handle/331001/1826]
专题	上海神经科学研究所_神经所(总)
推荐引用方式 GB/T 7714	Wang, LC,Xiong, W,Zheng, J,et al. The timing of endocytosis after activation of a G-protein-coupled receptor in a sensory neuron[J]. BIOPHYSICAL JOURNAL,2006,90(10):3590-3598.
APA	Wang, LC.,Xiong, W.,Zheng, J.,Zhou, Y.,Zheng, H.,...&Zhou, Z.(2006).The timing of endocytosis after activation of a G-protein-coupled receptor in a sensory neuron.BIOPHYSICAL JOURNAL,90(10),3590-3598.
MLA	Wang, LC,et al."The timing of endocytosis after activation of a G-protein-coupled receptor in a sensory neuron".BIOPHYSICAL JOURNAL 90.10(2006):3590-3598.