Severe acute respiratory syndrome-associated coronavirus 3a protein forms an ion channel and modulates virus release

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	Severe acute respiratory syndrome-associated coronavirus 3a protein forms an ion channel and modulates virus release
	Lu, Wei ; Zheng, Bo-Jian ; Xu, Ke ; Schwarz, Wolfgang ; Du, Lanying ; Wong, Charlotte K. L. ; Chen, Jiandong ; Duan, Shuming ; Deubel, Vincent ; Sun, Bing
刊名	PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
	2006
卷号	103 期号:33 页码:12540-12545
关键词	ORF 3a two-electrode voltage clamp tetramer channel activity SARS-ASSOCIATED CORONAVIRUS STRUCTURAL PROTEIN RNA INTERFERENCE EXPRESSING CELLS INFECTED-CELLS VPU PROTEIN M2 PROTEIN IDENTIFICATION APOPTOSIS HIV-1
ISSN号	0027-8424
通讯作者	Sun, B (reprint author), Chinese Acad Prevent Med, Inst Pasteur, Mol Virol Lab, 225 S Chongqing Rd, Shanghai 200025, Peoples R China,bsun@sibs.ac.cn
英文摘要	Fourteen ORFs have been identified in the severe acute respiratory syndrome-associated coronavirus (SARS-CoV) genome. ORF 3a of SARS-CoV codes for a recently identified transmembrane protein, but its function remains unknown. In this study we confirmed the 3a protein expression and investigated its localization at the surface of SARS-CoV-infected or 3a-cDNA-transfected cells. Our experiments showed that recombinant 3a protein can form a homotetramer complex through interprotein disulfide bridges in 3a-cDNA-transfected cells, providing a clue to ion channel function. The putative ion channel activity of this protein was assessed in 3a-complement RNA-injected Xenopus oocytes by two-electrode voltage clamp. The results suggest that 3a protein forms a potassium sensitive channel, which can be efficiently inhibited by barium. After FRhK-4 cells were transfected with an siRNA, which is known to suppress 3a expression, followed by infection with SARS-CoV, the released virus was significantly decreased, whereas the replication of the virus in the infected cells was not changed. Our observation suggests that SARS-CoV ORF 3a functions as an ion channel that may promote virus release. This finding will help to explain the highly pathogenic nature of SARS-CoV and to develop new strategies for treatment of SARS infection.
学科主题	Science & Technology - Other Topics
收录类别	SCI
语种	英语
公开日期	2012-07-23
内容类型	期刊论文
源URL	[http://ir.sibs.ac.cn/handle/331001/1807]
专题	上海神经科学研究所_神经所(总)
推荐引用方式 GB/T 7714	Lu, Wei,Zheng, Bo-Jian,Xu, Ke,et al. Severe acute respiratory syndrome-associated coronavirus 3a protein forms an ion channel and modulates virus release[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,2006,103(33):12540-12545.
APA	Lu, Wei.,Zheng, Bo-Jian.,Xu, Ke.,Schwarz, Wolfgang.,Du, Lanying.,...&Sun, Bing.(2006).Severe acute respiratory syndrome-associated coronavirus 3a protein forms an ion channel and modulates virus release.PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,103(33),12540-12545.
MLA	Lu, Wei,et al."Severe acute respiratory syndrome-associated coronavirus 3a protein forms an ion channel and modulates virus release".PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 103.33(2006):12540-12545.