Amiloride attenuates glycine-induced currents in cultured neurons of rat inferior colliculus

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	Amiloride attenuates glycine-induced currents in cultured neurons of rat inferior colliculus
	Tang, Zheng-Quan ; Lu, Yun-Gang ; Zhou, Ke-Qing ; Xu, Tian-Le ; Chen, Lin
刊名	BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
	2006
卷号	350 期号:4 页码:900-904
关键词	amiloride glycine receptor inferior colliculus whole-cell patch-clamp cell culture rat DORSAL-HORN NEURONS NA+ CHANNEL SPINAL NEURONS HAIR-CELLS RECEPTOR ANALOGS BRAIN INHIBITION TRANSDUCTION CONDUCTANCE
ISSN号	0006-291X
通讯作者	Chen, L (reprint author), Univ Sci & Technol China, Auditory Res Lab, Sch Life Sci, Hefei 230027, Peoples R China,linchen@ustc.edu.cn
英文摘要	Amiloride, a potassium sparing diuretic, is well known to interact with many ion transport systems and modulate the activity of several membrane receptors. However, relatively little information is available as to how amiloride affects membrane receptors of neurons in the brain areas. In the present study, we investigated the effects of amiloride on glycine-induced currents (I-Gly) in cultured neurons of rat inferior colliculus with whole-cell patch-clamp recordings. Amiloride itself did not activate any current across the neuronal membrane but it reversibly inhibited the amplitude of the I-Gly in a reversible and concentration-dependent manner, with an IC50 of 487.4 +/- 25.3 mu M (n = 5). Amiloride shifted the concentration-response relationship to the right without changing Hill coefficient and without changing the maximum response of the I-Gly. The pre-perfusion of amiloride produced an inhibitory effect on the I-Gly. In addition, amiloride was shown with a voltage ramp protocol to significantly reduce the conductance induced by glycine but not to change the reversal potential of the I-Gly. These results demonstrate that amiloride competitively inhibits the I-Gly in rat inferior colliculus neurons by decreasing the affinity of glycine to its receptor. Our finding suggests that attention should be paid to the possible side effects of amiloride used as a drug on brain functions in the case of a defective blood-brain barrier and in the case of direct application of this drug into the cerebrospinal fluid for treatment of brain tumors. (c) 2006 Elsevier Inc. All rights reserved.
学科主题	Biochemistry & Molecular Biology ; Biophysics
收录类别	SCI
语种	英语
公开日期	2012-07-23
内容类型	期刊论文
源URL	[http://ir.sibs.ac.cn/handle/331001/1798]
专题	上海神经科学研究所_神经所(总)
推荐引用方式 GB/T 7714	Tang, Zheng-Quan,Lu, Yun-Gang,Zhou, Ke-Qing,et al. Amiloride attenuates glycine-induced currents in cultured neurons of rat inferior colliculus[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2006,350(4):900-904.
APA	Tang, Zheng-Quan,Lu, Yun-Gang,Zhou, Ke-Qing,Xu, Tian-Le,&Chen, Lin.(2006).Amiloride attenuates glycine-induced currents in cultured neurons of rat inferior colliculus.BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,350(4),900-904.
MLA	Tang, Zheng-Quan,et al."Amiloride attenuates glycine-induced currents in cultured neurons of rat inferior colliculus".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 350.4(2006):900-904.