DISTRIBUTION OF ACID-SENSING ION CHANNEL 3 IN THE RAT HYPOTHALAMUS

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	DISTRIBUTION OF ACID-SENSING ION CHANNEL 3 IN THE RAT HYPOTHALAMUS
	Meng, Q. -Y. ; Wang, W. ; Chen, X. -N. ; Xu, T. -L. ; Zhou, J. -N.
刊名	NEUROSCIENCE
	2009
卷号	159 期号:3 页码:1126-1134
关键词	ASIC3 hypothalamus RT-PCR Western blot immunohistochemistry CENTRAL-NERVOUS-SYSTEM GATED CATION CHANNEL SENSORY NEURONS MOLECULAR-CLONING NA+ CHANNEL EXPRESSION BRAIN LOCALIZATION FAMILY ASIC3
ISSN号	0306-4522
通讯作者	Zhou, JN (reprint author), Univ Sci & Technol China, Sch Life Sci, Dept Neurobiol, Rd 433, Hefei 230027, Anhui, Peoples R China,jnzhou@ustc.edu.cn
英文摘要	Acid-sensing ion channels (ASICs), the members of the epithelial sodium channel/degenerin (ENaC/DEG) superfamily, are proton-gated voltage-insensitive cation channels. Six ASIC subunits have been identified and characterized in the mammalian nervous system so far. Of these subunits, ASIC3 has been shown to be predominantly expressed in the peripheral nervous system of rodents and implicated in mechnosensation, chemosensation and pain perception. Little is known on ASIC3 in the brain. We thus employed reverse transcription-polymerase chain reaction (RT-PCR) and Western blot to examine the expression of ASIC3 in various rat brain regions, including hippocampus, amygdala, caudate putamen, prefrontal cortex, and hypothalamus. Specific attention was paid to the distribution of ASIC3 in the hypothalamus of rats by using immunohistochemistry. ASIC3 immunoreactivity showed a widespread pattern throughout the hypothalamus, with the highest density in paraventricular nucleus, supraoptic nucleus, suprachiasmatic nucleus, arcuate nucleus, dorsomedial nucleus, median preoptic nucleus, ventromedial preoptic nucleus, and dorsal tuberomammillary nucleus. This study may contribute to the understanding of ASIC3 functions in the CNS. (C) 2009 IBRO. Published by Elsevier Ltd. All rights reserved.
学科主题	Neurosciences & Neurology
收录类别	SCI
资助信息	National Key Project for Basic Science of China[2006CB500705]; Natural Science Foundation of China[30530310]
语种	英语
公开日期	2012-07-13
内容类型	期刊论文
源URL	[http://ir.sibs.ac.cn/handle/331001/1668]
专题	上海神经科学研究所_神经所(总)
推荐引用方式 GB/T 7714	Meng, Q. -Y.,Wang, W.,Chen, X. -N.,et al. DISTRIBUTION OF ACID-SENSING ION CHANNEL 3 IN THE RAT HYPOTHALAMUS[J]. NEUROSCIENCE,2009,159(3):1126-1134.
APA	Meng, Q. -Y.,Wang, W.,Chen, X. -N.,Xu, T. -L.,&Zhou, J. -N..(2009).DISTRIBUTION OF ACID-SENSING ION CHANNEL 3 IN THE RAT HYPOTHALAMUS.NEUROSCIENCE,159(3),1126-1134.
MLA	Meng, Q. -Y.,et al."DISTRIBUTION OF ACID-SENSING ION CHANNEL 3 IN THE RAT HYPOTHALAMUS".NEUROSCIENCE 159.3(2009):1126-1134.