Calpain Activation by Wingless-type Murine Mammary Tumor Virus Integration Site Family, Member 5A (Wnt5a) Promotes Axonal Growth

CORC > 上海神经科学研究所 > 中国科学院上海生命科学研究院神经科学研究所 > 神经所(总)

	Calpain Activation by Wingless-type Murine Mammary Tumor Virus Integration Site Family, Member 5A (Wnt5a) Promotes Axonal Growth
	Luo, Zhen-Ge
刊名	JOURNAL OF BIOLOGICAL CHEMISTRY
	2011
卷号	286 期号:8 页码:6566-6576
关键词	PROTEIN-KINASE-C BETA-CATENIN NEURONAL POLARIZATION PROTEOLYTIC ACTIVITY MEMBRANE PROTRUSION FRIZZLED HOMOLOGS SIGNALING PATHWAY MAMMALIAN CORTEX CELL-MIGRATION CONE FORMATION
ISSN号	0021-9258
通讯作者	Luo, ZG (reprint author), Chinese Acad Sci, Inst Neurosci, State Key Lab Neurosci, Shanghai Inst Biol Sci, 320 Yue Yang Rd, Shanghai 200031, Peoples R China,zgluo@ion.ac.cn
英文摘要	Axon development involves spatial-temporal cytoskeletal reorganization. However, how the cytoskeleton remodeling is modulated by extracellular cues is unclear. Here, we report a role of Wnt/Ca(2+) signaling in regulating actin and growth cone dynamics. We found that treatment of cultured cortical neurons with Wnt5a, a non-canonical Wnt, either globally or locally, caused an increase in the activity of calpain, a calcium-dependent protease responsible for the cleavage of several actin binding proteins, including spectrin. Treatment with Wnt5a promoted growth cone advance, as well as axonal growth, and these effects were prevented by chelating intracellular calcium, inhibition or down-regulation of calpain, or blockade of spectrin cleavage by competitive peptides. Interestingly, both Wnt5a and activated calpain were found to be mainly distributed in the axon-rich intermediate zone of neocortex. Down-regulating calpain expression interfered with the growth of callosal axons in vivo. Thus, Wnt5a serves as a physiological cue to stimulate localized calpain activity, which in turn promotes growth cone advance and axonal growth.
学科主题	Biochemistry & Molecular Biology
收录类别	SCI
资助信息	National Natural Science Foundation of China[31021063, 30825013]; National Basic Research Program[2011CBA00403]; Chinese Academy of Sciences[KSCX2-YW-R-102]; Program of Shanghai Subject Chief Scientist[08XD14050]
语种	英语
公开日期	2012-07-13
内容类型	期刊论文
源URL	[http://ir.sibs.ac.cn/handle/331001/1551]
专题	上海神经科学研究所_神经所(总) 上海神经科学研究所_突触信号研究组
推荐引用方式 GB/T 7714	Luo, Zhen-Ge. Calpain Activation by Wingless-type Murine Mammary Tumor Virus Integration Site Family, Member 5A (Wnt5a) Promotes Axonal Growth[J]. JOURNAL OF BIOLOGICAL CHEMISTRY,2011,286(8):6566-6576.
APA	Luo, Zhen-Ge.(2011).Calpain Activation by Wingless-type Murine Mammary Tumor Virus Integration Site Family, Member 5A (Wnt5a) Promotes Axonal Growth.JOURNAL OF BIOLOGICAL CHEMISTRY,286(8),6566-6576.
MLA	Luo, Zhen-Ge."Calpain Activation by Wingless-type Murine Mammary Tumor Virus Integration Site Family, Member 5A (Wnt5a) Promotes Axonal Growth".JOURNAL OF BIOLOGICAL CHEMISTRY 286.8(2011):6566-6576.