c-Maf Is Required for the Development of Dorsal Horn Laminae III/IV Neurons and Mechanoreceptive DRG Axon Projections

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	c-Maf Is Required for the Development of Dorsal Horn Laminae III/IV Neurons and Mechanoreceptive DRG Axon Projections
	Cheng, Leping
刊名	JOURNAL OF NEUROSCIENCE
	2012
卷号	32 期号:16 页码:5362-5373
关键词	VESICULAR GLUTAMATE TRANSPORTERS SPINAL-CORD SENSORY NEURONS TRANSMITTER PHENOTYPES TRANSCRIPTION FACTORS PROPER DEVELOPMENT ROOT GANGLIA CELL FATE EXPRESSION GENES
ISSN号	0270-6474
通讯作者	Cheng, LP (reprint author), Chinese Acad Sci, Inst Neurosci, 320 Yue Yang Rd, Shanghai 200031, Peoples R China,lpcheng@ion.ac.cn
英文摘要	Establishment of proper connectivity between peripheral sensory neurons and their central targets is required for an animal to sense and respond to various external stimuli. Dorsal root ganglion(DRG) neurons convey sensory signals of different modalities via their axon projections to distinct laminae in the dorsal horn of the spinal cord. In this study, we found that c-Maf was expressed predominantly in the interneurons of laminae III/IV, which primarily receive inputs from mechanoreceptive DRG neurons. In the DRG, c-Maf(+) neurons also coexpressed neurofilament-200, a marker for the medium-and large-diameter myelinated afferents that transmit non-noxious information. Furthermore, mouse embryos deficient in c-Maf displayed abnormal development of dorsal horn laminae III/IV neurons, as revealed by the marked reduction in the expression of several marker genes for these neurons, including those for transcription factors MafA and Rora, GABA(A) receptor subunit alpha 5, and neuropeptide cholecystokinin. In addition, among the four major subpopulations of DRG neurons marked by expression of TrkA, TrkB, TrkC, and MafA/GFR alpha 2/Ret, c-Maf was required selectively for the proper differentiation of MafA (+)/Ret (+)/GFR alpha 2 (+) low-threshold mechano-receptors (LTMs). Last, we found that the central and peripheral projections of mechanoreceptive DRG neurons were compromised in c-Maf deletion mice. Together, our results indicate that c-Maf is required for the proper development of MafA (+)/Ret (+)/GFR alpha 2 (+) LTMs in the DRG, their afferent projections in the dorsal horn and Pacinian corpuscles, as well as neurons in laminae III/IV of the spinal cord.
学科主题	Neurosciences & Neurology
收录类别	SCI
资助信息	Ministry of Science and Technology of China[2011CBA00400]; Chinese Academy of Sciences[XDA01020306]; [2007CB947102]
语种	英语
公开日期	2012-07-13
内容类型	期刊论文
源URL	[http://ir.sibs.ac.cn/handle/331001/1490]
专题	上海神经科学研究所_神经所(总) 上海神经科学研究所_神经发育及其调控机理研究组
推荐引用方式 GB/T 7714	Cheng, Leping. c-Maf Is Required for the Development of Dorsal Horn Laminae III/IV Neurons and Mechanoreceptive DRG Axon Projections[J]. JOURNAL OF NEUROSCIENCE,2012,32(16):5362-5373.
APA	Cheng, Leping.(2012).c-Maf Is Required for the Development of Dorsal Horn Laminae III/IV Neurons and Mechanoreceptive DRG Axon Projections.JOURNAL OF NEUROSCIENCE,32(16),5362-5373.
MLA	Cheng, Leping."c-Maf Is Required for the Development of Dorsal Horn Laminae III/IV Neurons and Mechanoreceptive DRG Axon Projections".JOURNAL OF NEUROSCIENCE 32.16(2012):5362-5373.