RP-HPLC and NMR Study of Antioxidant Flavonoids in Extract from Gentiana piasezkii

CORC > 兰州化学物理研究所 > 中国科学院兰州化学物理研究所 > 中科院西北特色植物资源化学重点实验室/甘肃省天然药物重点实验室

	RP-HPLC and NMR Study of Antioxidant Flavonoids in Extract from Gentiana piasezkii
	Shi YP(师彦平); Chen J(陈娟); Shi YP(师彦平)
刊名	J. Anal. Chem.
	2010
卷号	65 期号:3 页码:298-304
ISSN号	1061-9348
通讯作者	师彦平
中文摘要	A reversed_phase high performance liquid chromatography (RP_HPLC) separation with photo_diode array detection (PDA) was developed for the simultaneous determination of five flavonoids, lutonarin(LO), saponarin (SA), isoorient (IO), 7_O_feruloylorientin (FO) and luteolin (LE) in the extract of Gentiana piasezkii, a popular forage grass, pharmaceutical and ornamental plant in China. The optimized method was achieved for the separation and detection of selected constituents, using acetonitrile_1% acetic acid as the mobile phase with gradient elution at a flow rate of 0.8 mL/min and 350 nm as the detection wavelength. The contents of the five flavonoids, i.e., LO, SA, IO, FO and LE in the plant of G. piasezkii were 0.1524, 0.0673, 0.300, 0.0552 and 0.0052%, respectively. The compounds possess similar structures and have been studied by nuclear magnetic resonance (NMR) spectroscopy.
学科主题	分析化学与药物化学
收录类别	SCI
资助信息	the National Basic Research Program (program 973) of China (2007CB108903);the National Natural Science Foundation of China (no. 20621091)
语种	英语
WOS记录号	WOS:000276714100015
公开日期	2012-09-29
内容类型	期刊论文
源URL	[http://210.77.64.217/handle/362003/1201]
专题	兰州化学物理研究所_中科院西北特色植物资源化学重点实验室/甘肃省天然药物重点实验室
通讯作者	Shi YP(师彦平); Shi YP(师彦平)
推荐引用方式 GB/T 7714	Shi YP,Chen J,Shi YP. RP-HPLC and NMR Study of Antioxidant Flavonoids in Extract from Gentiana piasezkii[J]. J. Anal. Chem.,2010,65(3):298-304.
APA	师彦平,陈娟,&师彦平.(2010).RP-HPLC and NMR Study of Antioxidant Flavonoids in Extract from Gentiana piasezkii.J. Anal. Chem.,65(3),298-304.
MLA	师彦平,et al."RP-HPLC and NMR Study of Antioxidant Flavonoids in Extract from Gentiana piasezkii".J. Anal. Chem. 65.3(2010):298-304.