Effect of salt treatment on theanine biosynthesis in Camellia sinensis seedlings

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	Effect of salt treatment on theanine biosynthesis in Camellia sinensis seedlings
	Deng, Wei-Wei 1,2; Wang, Shuo 1,2; Chen, Qi 1,2; Zhang, Zheng-Zhu 1,2; Hu, Xiang-Yang 3
刊名	PLANT PHYSIOLOGY AND BIOCHEMISTRY
	2012-07-01
卷号	56 期号:1 页码:35-40
关键词	Camellia sinensis Theanine synthetase Salt Theanine Amino acid
ISSN号	0981-9428
通讯作者	Zhang, ZZ (reprint author), Anhui Agr Univ, Key Lab Tea Biochem & Biotechnol, Minist Educ, 130 Changjiang W Rd, Hefei 230036, Anhui, Peoples R China. ; zzz@ahau.edu.cn
英文摘要	Theanine synthetase (TS) is an enzyme involved in theanine biosynthesis in tea plants. Recent studies have revealed that theanine biosynthesis, derived from nitrogen metabolism in tea (Camellia sinensis plants, could be influenced by salt treatment. We have characterized CsTS at the molecular and biochemical level. The expression pattern of CsTS protein was examined by western blot using a self-prepared polyclonal antibody with high specificity and sensitivity. The effect of salt treatment on the levels of theanine synthesis was investigated in this study. Levels of theanine and the total free amino acids were gradually increased in shoots, and reached the maximum on the 8th day after treatment (DAT). The immunoblotting analysis suggested the accumulation of CsTS protein had increased gently up to 8 DAT, and subsequently declined, both in roots and shoots, which is one of the main evidences that resulted in the variation of theanine concentration under salt treatment. Together, these data revealed that theanine synthesis takes place both in root and shoot and CsTS accumulation is positively affected by salt treatment. (C) 2012 Elsevier Masson SAS. All rights reserved.
学科主题	Plant Sciences
类目[WOS]	Plant Sciences
研究领域[WOS]	Plant Sciences
关键词[WOS]	ETHYLAMINE CONTENT ; L-ALANINE ; STRESS ; EXPRESSION ; ROOTS ; PLANT ; ACID
收录类别	SCI
资助信息	Program for Changjiang Scholars and Innovative Research Team in University[IRT1101]; Natural Science Foundation of China[30972400]
语种	英语
WOS记录号	WOS:000305779600004
公开日期	2012-09-03
内容类型	期刊论文
源URL	[http://ir.kib.ac.cn:8080/handle/151853/15117]
专题	昆明植物研究所_中国科学院青藏高原研究所昆明部
作者单位	1.Anhui Agr Univ, Key Lab Tea Biochem & Biotechnol, Minist Educ, Hefei 230036, Anhui, Peoples R China 2.Anhui Agr Univ, Minist Agr, Hefei 230036, Anhui, Peoples R China 3.Chinese Acad Sci, Key Lab Biodivers & Biogeog, Kunming Inst Bot, Inst Tibet Plateau Res Kunming, Kunming 650204, Peoples R China
推荐引用方式 GB/T 7714	Deng, Wei-Wei,Wang, Shuo,Chen, Qi,et al. Effect of salt treatment on theanine biosynthesis in Camellia sinensis seedlings[J]. PLANT PHYSIOLOGY AND BIOCHEMISTRY,2012,56(1):35-40.
APA	Deng, Wei-Wei,Wang, Shuo,Chen, Qi,Zhang, Zheng-Zhu,&Hu, Xiang-Yang.(2012).Effect of salt treatment on theanine biosynthesis in Camellia sinensis seedlings.PLANT PHYSIOLOGY AND BIOCHEMISTRY,56(1),35-40.
MLA	Deng, Wei-Wei,et al."Effect of salt treatment on theanine biosynthesis in Camellia sinensis seedlings".PLANT PHYSIOLOGY AND BIOCHEMISTRY 56.1(2012):35-40.