Determination of modified nucleosides in Escherichia coli by Liquid Chromatography-Electrospray Ionization Tandem Mass Spectrometry

	Determination of modified nucleosides in Escherichia coli by Liquid Chromatography-Electrospray Ionization Tandem Mass Spectrometry
	Xie YP(谢玉萍) ; Gao P(高鹏) ; Wang XY(王希越) ; Lian RW(练荣伟) ; Tian J(田晶) ; Xu GW(许国旺)
	2011
会议名称	37th international symposium on high performance liquid phase separations and related techniques
会议日期	2011-10-8
会议地点	大连
页码	503-0
通讯作者	许国旺
中文摘要	to date, there are about 100 modified nucleosides (mns) have been identified in rna species and the majority of them were found in trnas. except their common properties as components of rnas, mns have been confirmed to be of multifunctional. some researches had demonstrated mns’ involvement in anti-stress responses in the yeast. gene yfcc was recently found to modulate anaerobic survival of escherichia. coli (e. coli) by us. in order to determine if disruption of yfcc could stimulate certain response manifesting by mns, we developed a quick and simple method to quantify 20 nucleosides by using triple quadurupole liquid chromatography-tandem mass spectrometry (lc-ms/ms). based on the consideration of the polar properties of the mns, an acquity uplc hss t3 column was adopted with the mobile phase of 10 mm ammonium acetate and acetonitrile at a flow-rate of 0.2 ml min-1. for the improvement of detection limit, multiple reaction monitoring mode as well as positive electrospray ionization was employed by using triple quadrupole mass spectrometry. the linearity of the detected mns was excellent with r2 > 0.99 and the limits of detection were satisfied. the intra- and inter-day variation was ranged from 1.5% to 5.6% and 2.6% to 18.4%. many of the included nuclesides could be readily quantified in e. coli and the most abundant of which was inosine. additionally, distinct composition of the mns could be distinguished between the wild type and genetic modified strains. this study indicated the effects of genetic manipulation on mns in prokaryote also existed.
合作状况	墙报
会议主办者	中国化学会色谱专业委员会
会议录	proceeding of hplc 2011
会议录出版者	待补充
会议录出版地	待补充
学科主题	分析化学
内容类型	会议论文
源URL	[http://159.226.238.44/handle/321008/116079]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
推荐引用方式 GB/T 7714	Xie YP,Gao P,Wang XY,et al. Determination of modified nucleosides in Escherichia coli by Liquid Chromatography-Electrospray Ionization Tandem Mass Spectrometry[C]. 见:37th international symposium on high performance liquid phase separations and related techniques. 大连. 2011-10-8.