Metabolic profiling based on LC-MS to evaluate unintended effects of

	Metabolic profiling based on LC-MS to evaluate unintended effects of
	Chang YW(常玉玮) ; Zhao CX(赵春霞) ; Wu ZM(吴泽明) ; Zhou J(周佳) ; Zhao YN(赵燕妮) ; Lu X(路鑫) ; Xu GW(许国旺)
	2011
会议名称	37th international symposium on high performance liquid phase separations and related techniques
会议日期	2011-10-8
会议地点	大连
页码	484-0
通讯作者	许国旺
中文摘要	genetially modified (gm) plants have been widely used in agriculture and food industry recently. safety evaluation of gm plants for humans and environment has been becoming a more important topic although there has been no direct evidence indicating deleterious effect of gm plants. more and more studies have been carried out to explore differences in gm rice and their wild counterpart to evaluate unintended effect, which is a main aspect of the safety assessment. most of work was only focused on key nutrients and antinutrients. however, to the best of our knowledge metabolic profiling analysis of rice seed using lc-ms has not ever been reported. here a metabolic profiling method of rice seeds was developed using rapid resolution liquid chromatography/quadrupole time-of-flight mass spectrometry. the analytical properties of the method including the linearity, reproducibility, intra-day precision and inter-day precision were investigated and results were satisfactory. the method was then applied to investigate the differences between the transgenic rice and wild counterpart, as well as the wild rice with different sowing dates or sites. global metabolic phenotype differences were visualized and metabolites in discriminating different groups were found by a multivariate data analysis. results indicated that environment factors play a greater role than the gene modification for most of metabolites.
合作状况	墙报
会议主办者	中国化学会色谱专业委员会
会议录	proceeding of hplc 2011
会议录出版者	待补充
会议录出版地	待补充
学科主题	分析化学
内容类型	会议论文
源URL	[http://159.226.238.44/handle/321008/116067]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
推荐引用方式 GB/T 7714	Chang YW,Zhao CX,Wu ZM,et al. Metabolic profiling based on LC-MS to evaluate unintended effects of[C]. 见:37th international symposium on high performance liquid phase separations and related techniques. 大连. 2011-10-8.