Genetic, proteomic and metabolic analysis of the regulation of energy storage in rice seedlings in response to drought | |
Shu, Liebo1,3; Lou, Qiaojun1; Ma, Chenfei2; Ding, Wei1; Zhou, Jia2; Wu, Jinhong1; Feng, Fangjun1; Lu, Xin2; Luo, Lijun1,3; Xu, Guowang2 | |
刊名 | proteomics |
2011-11-01 | |
卷号 | 11期号:21页码:4122-4138 |
关键词 | cDNA microarray Drought-induced stress Metabolites Oryza sativa L. Plant proteomics |
ISSN号 | 待补充 |
通讯作者 | 许国旺 |
产权排序 | 2,3 |
中文摘要 | genetic, proteomic and metabolic analysis of the regulation of energy storage in rice seedlings in response to drought |
英文摘要 | we used proteomic analysis to determine the response of rice plant seedlings to drought-induced stress. the expression of 71 protein spots was significantly altered, and 60 spots were successfully identified. the greatest down-regulated protein functional category was translation. up-regulated proteins were mainly related to protein folding and assembly. additionally, many proteins involved in metabolism (e. g. carbohydrate metabolism) also showed differences in expression. cdna microarray and gc-ms analysis showed 4756 differentially expressed mrnas and 37 differentially expressed metabolites. once these data were integrated with the proteomic analysis, we were able to elucidate the metabolic pathways affected by drought-induced stress. these results suggest that increased energy consumption from storage substances occurred during drought. in addition, increased expression of the enzymes involved in anabolic pathways corresponded with an increase in the content of six amino acids. we speculated that energy conversion from carbohydrates and/or fatty acids to amino acids was increased. analysis of basic metabolism networks allowed us to understand how rice plants adjust to drought conditions. |
学科主题 | 物理化学 |
WOS标题词 | science & technology ; life sciences & biomedicine |
类目[WOS] | biochemical research methods ; biochemistry & molecular biology |
研究领域[WOS] | biochemistry & molecular biology |
关键词[WOS] | oryza-sativa l. ; transgenic arabidopsis plants ; sucrose-phosphate synthase ; water-stress ; carbohydrate-metabolism ; abiotic stress ; maize leaves ; protein network ; osmotic-stress ; heat-shock |
收录类别 | SCI |
语种 | 英语 |
WOS记录号 | WOS:000297223200002 |
公开日期 | 2012-07-09 |
内容类型 | 期刊论文 |
源URL | [http://159.226.238.44/handle/321008/115674] |
专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
作者单位 | 1.Shanghai Agrobiol Gene Ctr, Shanghai 201106, Peoples R China 2.Chinese Acad Sci, Dalian Inst Chem Phys, CAS Key Lab Separat Sci Analyt Chem, Dalian, Peoples R China 3.Huazhong Agr Univ, Natl Key Lab Crop Genet Improvement, Wuhan, Peoples R China |
推荐引用方式 GB/T 7714 | Shu, Liebo,Lou, Qiaojun,Ma, Chenfei,et al. Genetic, proteomic and metabolic analysis of the regulation of energy storage in rice seedlings in response to drought[J]. proteomics,2011,11(21):4122-4138. |
APA | Shu, Liebo.,Lou, Qiaojun.,Ma, Chenfei.,Ding, Wei.,Zhou, Jia.,...&Mei, Hanwei.(2011).Genetic, proteomic and metabolic analysis of the regulation of energy storage in rice seedlings in response to drought.proteomics,11(21),4122-4138. |
MLA | Shu, Liebo,et al."Genetic, proteomic and metabolic analysis of the regulation of energy storage in rice seedlings in response to drought".proteomics 11.21(2011):4122-4138. |
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