Effect of the traditional Chinese medicine tongxinluo on endothelial dysfunction rats studied by using urinary metabonomics based on liquid chromatography-mass spectrometry

	Effect of the traditional Chinese medicine tongxinluo on endothelial dysfunction rats studied by using urinary metabonomics based on liquid chromatography-mass spectrometry
	Dai, Weidong 2; Wei, Cong 1; Kong, Hongwei 2; Jia, Zhenhua 1; Han, Jianke 1; Zhang, Fengxia 2; Wu, Zeming 2; Gu, Yan 2; Chen, Shili 2; Gu, Qun 2
刊名	journal of pharmaceutical and biomedical analysis
	2011-08-25
卷号	56 期号:1 页码:86-92
关键词	Metabonomics Urine Endothelial dysfunction LC-MS Tongxinluo Simvastatin
ISSN号	待补充
通讯作者	wuyiling ; 许国旺
产权排序	1,1
中文摘要	effect of the traditional chinese medicine tongxinluo on endothelial dysfunction rats studied by using urinary metabonomics based on liquid chromatography-mass spectrometry
英文摘要	a urinary metabonomic method based on ultra-fast liquid chromatography coupled with ion trap-time of flight mass spectrometry (uflc/ms-it-tof) was employed to study the preventive efficacy and the metabolic changes caused by simavastatin and the traditional chinese medicine tongxinluo in endothelial dysfunction rats. principal component analysis (pca) was applied to study metabolic patterns of endothelial dysfunction rats and healthy control rats. 1-methyladenosine, indoxyl sulfate, hippuric acid, riboflavin, coproporphyrin, and p-cresol glucuronide were identified as potential biomarkers, indicating that pathways of adenine, tryptophan, phenylalanine, riboflavin, and porphyrin metabolism were disturbed in endothelial dysfunction rats. applications of simvastatin and tongxinluo to endothelial dysfunction rats improved endothelial function according to the results of histopathology and measurements of endothelin-1 and nitric oxide. metabonomic studies suggested that tongxinluo prevents endothelial dysfunction by regulating multiple metabolic pathways to their normal state, whereas simvastatin only altered selected metabolic pathways. this research demonstrated that metabonomics is a powerful and promising tool for disease investigation and the efficacy evaluation of complex traditional chinese medicines. (c) 2011 elsevier b.v. all rights reserved.
学科主题	物理化学
WOS标题词	science & technology ; physical sciences ; life sciences & biomedicine
类目[WOS]	chemistry, analytical ; pharmacology & pharmacy
研究领域[WOS]	chemistry ; pharmacology & pharmacy
关键词[WOS]	indoxyl sulfate ; oxidative stress ; uremic toxin ; p-cresol ; hplc-ms ; cells ; atherosclerosis ; metabolomics ; strategy ; systems
收录类别	SCI
语种	英语
WOS记录号	WOS:000291913500012
公开日期	2012-07-09
内容类型	期刊论文
源URL	[http://159.226.238.44/handle/321008/115670]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
作者单位	1.Integrat Tradit & Western Med Res Acad Hebei Prov, Shijiazhuang 050035, Hebei Province, Peoples R China 2.Chinese Acad Sci, Dalian Inst Chem Phys, CAS Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
推荐引用方式 GB/T 7714	Dai, Weidong,Wei, Cong,Kong, Hongwei,et al. Effect of the traditional Chinese medicine tongxinluo on endothelial dysfunction rats studied by using urinary metabonomics based on liquid chromatography-mass spectrometry[J]. journal of pharmaceutical and biomedical analysis,2011,56(1):86-92.
APA	Dai, Weidong.,Wei, Cong.,Kong, Hongwei.,Jia, Zhenhua.,Han, Jianke.,...&Xu, Guowang.(2011).Effect of the traditional Chinese medicine tongxinluo on endothelial dysfunction rats studied by using urinary metabonomics based on liquid chromatography-mass spectrometry.journal of pharmaceutical and biomedical analysis,56(1),86-92.
MLA	Dai, Weidong,et al."Effect of the traditional Chinese medicine tongxinluo on endothelial dysfunction rats studied by using urinary metabonomics based on liquid chromatography-mass spectrometry".journal of pharmaceutical and biomedical analysis 56.1(2011):86-92.