Hainanenins: A novel family of antimicrobial peptides with strong activity from Hainan cascade-frog, Amolops hainanensis

CORC > 烟台海岸带研究所 > 中国科学院烟台海岸带研究所 > 海岸带生物学与生物资源利用重点实验室 > 海岸带生物学与生物资源利用所重点实验室

	Hainanenins: A novel family of antimicrobial peptides with strong activity from Hainan cascade-frog, Amolops hainanensis
	Zhang, Songyan 1; Guo, Huanhuan 3; Shi, Fei 2,4; Wang, Hui3 ; Li, Lili2 ; Jiao, Xudong2 ; Wang, Yipeng2 ; Yu, Haining 1,3
刊名	PEPTIDES
	2012-02-01
卷号	33 期号:2 页码:251-257
关键词	Amphibian Antimicrobial peptide (AMP) Hainanenins Amolops hainanensis
ISSN号	0196-9781
通讯作者	Wang, YP (reprint author), Chinese Acad Sci, Yantai Inst Coastal Zone Res, Biol Resources Lab, Yantai 264003, Shandong, Peoples R China. wyp010@163.com ; joannyu@live.cn
产权排序	[Zhang, Songyan; Yu, Haining] Dalian Univ Technol, Dept Biosci & Biotechnol, Dalian 116023, Peoples R China; [Shi, Fei; Li, Lili; Jiao, Xudong; Wang, Yipeng] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Biol Resources Lab, Yantai 264003, Shandong, Peoples R China; [Guo, Huanhuan; Wang, Hui; Yu, Haining] Hebei Normal Univ, Coll Life Sci, Shijiazhuang 050016, Peoples R China; [Shi, Fei] Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China
中文摘要	Antimicrobial peptides (AMPs) secreted by amphibian skin represent an important innate immune defense strategy. There are more than 340 species in the family of Ranidae worldwidely, and from which nearly 100 families of AMPs comprising between 8 and 48 amino acid (aa) residues have been characterized. In current work, two novel AMPs were purified from the skin secretion of Hainan cascade-frog, Amolops hainanensis, and 31 cDNA sequences encoding 10 novel AMPs belonging to 4 families were cloned from the constructed skin cDNA library of A. hainanensis. Among these 10 AMPs, 5 peptides represent the prototypes of a novel amphibian AMP family. According to the generic name of the species of origin, they were designated as hainanenin-1-5. Each of them consists of 21 aa residues with a C-terminal disulphide loop of 7 residues between Cys(15) and Cys(21). Two of them (hainanenin-1 and 5) were then synthesized and their in vitro activities were screened, including antimicrobial, hemolytic and antioxidant activities. The results showed that hainanenin-1 and 5 possessed strong and broad-spectrum antimicrobial activities against Gram-positive, Gram-negative bacteria and fungi, including a large number of clinically isolated drug-resistant pathogenic microorganisms, and slight antioxidant activity. Undesirably, hainanenin-1 and 5 exhibited strong hemolytic activity on human erythrocytes. The discovery of hainanenins and their great antimicrobial potency provides new templates for anti-infective agent design. Crown Copyright
英文摘要	Antimicrobial peptides (AMPs) secreted by amphibian skin represent an important innate immune defense strategy. There are more than 340 species in the family of Ranidae worldwidely, and from which nearly 100 families of AMPs comprising between 8 and 48 amino acid (aa) residues have been characterized. In current work, two novel AMPs were purified from the skin secretion of Hainan cascade-frog, Amolops hainanensis, and 31 cDNA sequences encoding 10 novel AMPs belonging to 4 families were cloned from the constructed skin cDNA library of A. hainanensis. Among these 10 AMPs, 5 peptides represent the prototypes of a novel amphibian AMP family. According to the generic name of the species of origin, they were designated as hainanenin-1-5. Each of them consists of 21 aa residues with a C-terminal disulphide loop of 7 residues between Cys(15) and Cys(21). Two of them (hainanenin-1 and 5) were then synthesized and their in vitro activities were screened, including antimicrobial, hemolytic and antioxidant activities. The results showed that hainanenin-1 and 5 possessed strong and broad-spectrum antimicrobial activities against Gram-positive, Gram-negative bacteria and fungi, including a large number of clinically isolated drug-resistant pathogenic microorganisms, and slight antioxidant activity. Undesirably, hainanenin-1 and 5 exhibited strong hemolytic activity on human erythrocytes. The discovery of hainanenins and their great antimicrobial potency provides new templates for anti-infective agent design. Crown Copyright (C) 2012 Published by Elsevier Inc. All rights reserved.
学科主题	Biochemistry & Molecular Biology; Pharmacology & Pharmacy
研究领域[WOS]	Biochemistry & Molecular Biology ; Pharmacology & Pharmacy
关键词[WOS]	SPOTTED TORRENT FROG ; SKIN SECRETIONS ; AMPHIBIAN SKIN ; PEPTIDOMICS ; INHIBITORS ; LOLOENSIS ; SYSTEM
收录类别	SCI
资助信息	Chinese National Natural Science Foundation[30900240, 41076098]
原文出处	http://www.sciencedirect.com/science/journal/01969781
语种	英语
WOS记录号	WOS:000301871200009
公开日期	2012-06-13
内容类型	期刊论文
源URL	[http://ir.yic.ac.cn/handle/133337/5558]
专题	烟台海岸带研究所_海岸带生物学与生物资源利用所重点实验室
作者单位	1.Dalian Univ Technol, Dept Biosci & Biotechnol, Dalian 116023, Peoples R China 2.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Biol Resources Lab, Yantai 264003, Shandong, Peoples R China 3.Hebei Normal Univ, Coll Life Sci, Shijiazhuang 050016, Peoples R China 4.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Songyan,Guo, Huanhuan,Shi, Fei,et al. Hainanenins: A novel family of antimicrobial peptides with strong activity from Hainan cascade-frog, Amolops hainanensis[J]. PEPTIDES,2012,33(2):251-257.
APA	Zhang, Songyan.,Guo, Huanhuan.,Shi, Fei.,Wang, Hui.,Li, Lili.,...&Yu, Haining.(2012).Hainanenins: A novel family of antimicrobial peptides with strong activity from Hainan cascade-frog, Amolops hainanensis.PEPTIDES,33(2),251-257.
MLA	Zhang, Songyan,et al."Hainanenins: A novel family of antimicrobial peptides with strong activity from Hainan cascade-frog, Amolops hainanensis".PEPTIDES 33.2(2012):251-257.