Poly(o-phenylenediamine) Colloid-Quenched Fluorescent Oligonucleotide as a Probe for Fluorescence-Enhanced Nucleic Acid Detection

	Poly(o-phenylenediamine) Colloid-Quenched Fluorescent Oligonucleotide as a Probe for Fluorescence-Enhanced Nucleic Acid Detection
	Tian JQ ; Li HL ; Luo YL ; Wang L ; Zhang YW ; Sun XP
刊名	langmuir
	2011
卷号	27 期号:3 页码:874-877
关键词	MOLECULAR BEACONS DNA ANALYSIS HYBRIDIZATION GRAPHENE BIOMOLECULES PLATFORM PROTON
ISSN号	0743-7463
通讯作者	sun xp
中文摘要	in this letter, we demonstrate that chemical oxidation polymerization of o-phenylenediamine (opd) by potassium bichromate at room temperature results in the formation of submicrometer-scale poly(o-phenylenediamine) (popd) colloids. such colloids can absorb and quench dye-labeled single-stranded dna (ssdna) very effectively. in the presence of a target, a hybridization event occurs, which produces a double-stranded dna (dsdna) that detaches from the popd surface, leading to recovery of dye fluorescence. with the use of an oligonucleotide (ond) sequence associated with human immunodeficiency virus (hiv) as a model system, we demonstrate the proof of concept that popd colloid-quenched fluorescent ond can be used as a probe for fluorescence-enhanced nucleic acid detection with selectivity down to single-base mismatch.
收录类别	SCI收录期刊论文
语种	英语
WOS记录号	WOS:000286485600004
公开日期	2012-06-11
内容类型	期刊论文
源URL	[http://ir.ciac.jl.cn/handle/322003/44859]
专题	长春应用化学研究所_长春应用化学研究所知识产出_期刊论文
推荐引用方式 GB/T 7714	Tian JQ,Li HL,Luo YL,et al. Poly(o-phenylenediamine) Colloid-Quenched Fluorescent Oligonucleotide as a Probe for Fluorescence-Enhanced Nucleic Acid Detection[J]. langmuir,2011,27(3):874-877.
APA	Tian JQ,Li HL,Luo YL,Wang L,Zhang YW,&Sun XP.(2011).Poly(o-phenylenediamine) Colloid-Quenched Fluorescent Oligonucleotide as a Probe for Fluorescence-Enhanced Nucleic Acid Detection.langmuir,27(3),874-877.
MLA	Tian JQ,et al."Poly(o-phenylenediamine) Colloid-Quenched Fluorescent Oligonucleotide as a Probe for Fluorescence-Enhanced Nucleic Acid Detection".langmuir 27.3(2011):874-877.