Lectin Conjugated Gold Nanoparticle-based Colorimetric Assay for Studying the Interactions of Antibiotic with Living Cell

	Lectin Conjugated Gold Nanoparticle-based Colorimetric Assay for Studying the Interactions of Antibiotic with Living Cell
	Wang JE ; Wang CK ; Liu DJ ; Wang ZX
刊名	chemical research in chinese universities
	2011
卷号	27 期号:2 页码:193-197
关键词	N-LINKED OLIGOSACCHARIDES TUNICAMYCIN GLYCOSYLATION METASTASIS INHIBITION PROTEINS
ISSN号	1005-9040
通讯作者	wang zx
中文摘要	the interactions of antibiotic with living cells were studied by lectin conjugated gold nanoparticles(gnps) based colorimetric assay. because of the high affinity of lectin for saccharides, the lectin conjugated gnps are able to employ as indicators for monitoring the antibiotic induced changes of glycosyl complexes. the interactions of a well known antibiotic, tunicamycin, with two different cell lines, hela and shg-44, were selected to establish this assay. in the presence of tunicamycin, the dose- and time-dependence on the decreasing of binding affinity of lectin conjugated gnps with living cells were demonstrated by conventional microscopic and uv-vis spectroscopic studies. the experimental result demonstrates that our approach can be used to identify antibiotic induced expression difference of glycosyl complexes on different cellular surfaces and determine drug activity quantitatively. for further confirming the capability of the gnp-based assay, the system was also studied by confocal laser scanning microscopy(clsm) and classic flow cytometry(fcm) assay, and satisfactory results were obtained.
收录类别	SCI收录期刊论文
语种	英语
WOS记录号	WOS:000290001800009
公开日期	2012-06-11
内容类型	期刊论文
源URL	[http://ir.ciac.jl.cn/handle/322003/44775]
专题	长春应用化学研究所_长春应用化学研究所知识产出_期刊论文
推荐引用方式 GB/T 7714	Wang JE,Wang CK,Liu DJ,et al. Lectin Conjugated Gold Nanoparticle-based Colorimetric Assay for Studying the Interactions of Antibiotic with Living Cell[J]. chemical research in chinese universities,2011,27(2):193-197.
APA	Wang JE,Wang CK,Liu DJ,&Wang ZX.(2011).Lectin Conjugated Gold Nanoparticle-based Colorimetric Assay for Studying the Interactions of Antibiotic with Living Cell.chemical research in chinese universities,27(2),193-197.
MLA	Wang JE,et al."Lectin Conjugated Gold Nanoparticle-based Colorimetric Assay for Studying the Interactions of Antibiotic with Living Cell".chemical research in chinese universities 27.2(2011):193-197.