Conjugation of alpha-chymotrypsin on a polymeric hydrophilic nanolayer covering magnetic nanoparticles

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	Conjugation of alpha-chymotrypsin on a polymeric hydrophilic nanolayer covering magnetic nanoparticles
	Hong, J（洪军） ; Gong, PJ（龚培军） ; Yu, JH ; Xu, DM（徐冬梅） ; Sun, HW（孙汉文） ; Yao, S（姚思德）
刊名	JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC
	2006
卷号	42 期号:40606 页码:99
关键词	immobilized enzyme photochemical in situ polymerization alpha-chymotrypsin magnetic nanogel
ISSN号	1381-1177
通讯作者	Yao, SD (reprint author), Chinese Acad Sci, Shanghai Inst Appl Phys, Shanghai 201800, Peoples R China
英文摘要	Magnetic nanoparticles, covered by a polymeric hydrophilic nanolayer containing reactive amino groups, were obtained via Hoffman degradation of the polyacrylamide-coated Fe3O4 nanoparticles synthesized by photochemical in situ polymerization, and then conjugated the model enzyme-alpha-chymotrypsin (CT) by use of EDC. HCl and NHS at room temperatures. The mechanism of photochemical in situ polymerization was briefly proposed in this paper. Superparamagnetic properties were retained for Fe3O4 after enzyme immobilization while slightly reducing the value of saturation magnetization. Crystalline structure of Fe3O4 after CT immobilization was consistent with that of the freshly prepared Fe3O4 by X-ray diffraction (XRD) analysis. The binding capacity was 69 and 61 mg enzyme/g nanogel determined by thermogravimetric (TG) analysis and by standard BCA protein assay, respectively. Specific activity of the immobilized CT was 0.93 U/(mg min), only 59.3% as that of free CT. Thermal stability of CT was improved after being bound to the amine-functionalized magnetic nanogel. (c) 2006 Elsevier B.V. All rights reserved.
学科主题	Chemistry
收录类别	SCI
语种	英语
WOS记录号	WOS:000241660700006
公开日期	2012-05-03
内容类型	期刊论文
源URL	[http://ir.sinap.ac.cn/handle/331007/8025]
专题	上海应用物理研究所_中科院上海应用物理研究所2004-2010年
推荐引用方式 GB/T 7714	Hong, J,Gong, PJ,Yu, JH,et al. Conjugation of alpha-chymotrypsin on a polymeric hydrophilic nanolayer covering magnetic nanoparticles[J]. JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC,2006,42(40606):99.
APA	Hong, J,Gong, PJ,Yu, JH,Xu, DM,Sun, HW,&Yao, S.(2006).Conjugation of alpha-chymotrypsin on a polymeric hydrophilic nanolayer covering magnetic nanoparticles.JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC,42(40606),99.
MLA	Hong, J,et al."Conjugation of alpha-chymotrypsin on a polymeric hydrophilic nanolayer covering magnetic nanoparticles".JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC 42.40606(2006):99.