Inhibition of the In Vitro Replication of DNA by an Aptamer-Protein Complex in an Autonomous DNA Machine

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	Inhibition of the In Vitro Replication of DNA by an Aptamer-Protein Complex in an Autonomous DNA Machine
	Zhu, CF ; Wen, YQ ; Li, D(李迪） ; Wang, LH（王丽华） ; Song, SP（宋世平） ; Fan, CH（樊春海) ; Willner, I
刊名	CHEMISTRY-A EUROPEAN JOURNAL
	2009
卷号	15 期号:44 页码:11898
ISSN号	0947-6539
通讯作者	Li, D (reprint author), Chinese Acad Sci, Phys Biol Lab, Shanghai Inst Appl Phys, Shanghai 201800, Peoples R China
英文摘要	DNA replication plays a central role in living organisms. Unregulated or uncontrollable DNA replication is well known to result in many pathological states, such as cancer, autoimmune diseases, and viral/bacterial infections. We report that an aptamer-protein complex could indirectly inhibit in vitro replication of DNA. An isothermal DNA machine based on the strand-displacement amplification is employed to support our assumption. An antithrombin aptamer sequence is rationally encoded into the DNA replication template. Once thrombin binds to the template, the as-formed aptamer-protein complexes can, in turn, become a barrier to the polymerase and inhibit the DNA replication activities in both static and dynamic modes. The inhibition is successfully confirmed by both fluorescence and gel-electrophoresis experiments. Considering the availability of a broad library of aptamers and the existence of various DNA/protein interactions, our results imply the possibility for the rational regulation of DNA replication in vivo.
学科主题	Chemistry
收录类别	SCI
语种	英语
WOS记录号	WOS:000272109100015
公开日期	2012-04-18
内容类型	期刊论文
源URL	[http://ir.sinap.ac.cn/handle/331007/7753]
专题	上海应用物理研究所_中科院上海应用物理研究所2004-2010年
推荐引用方式 GB/T 7714	Zhu, CF,Wen, YQ,Li, D,et al. Inhibition of the In Vitro Replication of DNA by an Aptamer-Protein Complex in an Autonomous DNA Machine[J]. CHEMISTRY-A EUROPEAN JOURNAL,2009,15(44):11898.
APA	Zhu, CF.,Wen, YQ.,Li, D.,Wang, LH.,Song, SP.,...&Willner, I.(2009).Inhibition of the In Vitro Replication of DNA by an Aptamer-Protein Complex in an Autonomous DNA Machine.CHEMISTRY-A EUROPEAN JOURNAL,15(44),11898.
MLA	Zhu, CF,et al."Inhibition of the In Vitro Replication of DNA by an Aptamer-Protein Complex in an Autonomous DNA Machine".CHEMISTRY-A EUROPEAN JOURNAL 15.44(2009):11898.