Electron transfer reactivity and catalytic activity of structurally rigidized hemoglobin

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	Electron transfer reactivity and catalytic activity of structurally rigidized hemoglobin
	Wang, J ; Liang, ZQ ; Wang, LH ; Fan, CH ; Li, GX
刊名	SENSORS AND ACTUATORS B-CHEMICAL
	2007
卷号	125 期号:1 页码:17
ISSN号	0925-4005
通讯作者	Li, GX (reprint author), Nanjing Univ, Dept Biochem, Nanjing 210093, Peoples R China
英文摘要	A simple and novel strategy to obtain the direct electrochemistry of hemoglobin (Hb) was reported by enhancing its structural rigidity, which was demonstrated by the great promotion of the electron-transfer reactivity of the protein and its catalytic activity toward hydrogen peroxide (H2O2). Direct fast electron transfer between this structural rigidity enhanced Hb (SRE Hb) and a pyrolytic graphite (PG) electrode was achieved, and a pair of well-defined, quasi-reversible redox peaks were obtained. The cathodic and anodic peaks were located at -0.370 and -0.290 V, respectively. Meanwhile, the catalytic activity of the protein toward the reduction of H2O2 was also studied, and a H2O2 biosensor was subsequently prepared. Its detection limit was 3.4 x 10(-7) M with a sensitivity of 0.0224 mu A per mu M of H2O2. The apparent Michaelis-Menten constant was calculated to be 107 mu M, which was lower than those of many horseradish peroxidase based H2O2 sensors, suggesting that structural rigidity of Hb could endow the protein a high peroxidase activity. (c) 2007 Elsevier B.V. All rights reserved.
学科主题	Chemistry; Nuclear Science & Technology
收录类别	SCI
语种	英语
WOS记录号	WOS:000248516100003
公开日期	2012-04-18
内容类型	期刊论文
源URL	[http://ir.sinap.ac.cn/handle/331007/7715]
专题	上海应用物理研究所_中科院上海应用物理研究所2004-2010年
推荐引用方式 GB/T 7714	Wang, J,Liang, ZQ,Wang, LH,et al. Electron transfer reactivity and catalytic activity of structurally rigidized hemoglobin[J]. SENSORS AND ACTUATORS B-CHEMICAL,2007,125(1):17.
APA	Wang, J,Liang, ZQ,Wang, LH,Fan, CH,&Li, GX.(2007).Electron transfer reactivity and catalytic activity of structurally rigidized hemoglobin.SENSORS AND ACTUATORS B-CHEMICAL,125(1),17.
MLA	Wang, J,et al."Electron transfer reactivity and catalytic activity of structurally rigidized hemoglobin".SENSORS AND ACTUATORS B-CHEMICAL 125.1(2007):17.