Adenosine detection by using gold nanoparticles and designed aptamer sequences

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	Adenosine detection by using gold nanoparticles and designed aptamer sequences
	Li, F ; Zhang, J ; Cao, XN ; Wang, LH(王丽华） ; Li, D（李迪） ; Song, SP（宋世平） ; Ye, BC ; Fan, CH（樊春海）
刊名	ANALYST
	2009
卷号	134 期号:7 页码:1355
ISSN号	0003-2654
通讯作者	Wang, LH (reprint author), Chinese Acad Sci, Shanghai Inst Appl Phys, Shanghai 201800, Peoples R China
英文摘要	Based on gold nanoparticles (AuNPs) and engineered DNA aptamers, we designed a novel bioassay strategy for the detection of adenosine as a small target molecule. In this design, an aptamer is engineered to consist of two pieces of random-coil like ssDNA which are respectively attached to AuNPs through their 50-thiol-modified end. They can reassemble into the intact aptamer tertiary structure and induce nanoparticle aggregation in the presence of the specific target. Results have demonstrated that gold nanoparticles can effectively differentiate these two different DNA structures via their characteristic surface plasmon resonance-based color change. With this method, adenosine can be selectively detected in the low micromolar range, which means that the strategy reported here can be applicable to the detection of several other small target molecules.
学科主题	Chemistry
收录类别	SCI
语种	英语
WOS记录号	WOS:000267499800014
公开日期	2012-04-18
内容类型	期刊论文
源URL	[http://ir.sinap.ac.cn/handle/331007/7641]
专题	上海应用物理研究所_中科院上海应用物理研究所2004-2010年
推荐引用方式 GB/T 7714	Li, F,Zhang, J,Cao, XN,et al. Adenosine detection by using gold nanoparticles and designed aptamer sequences[J]. ANALYST,2009,134(7):1355.
APA	Li, F.,Zhang, J.,Cao, XN.,Wang, LH.,Li, D.,...&Fan, CH.(2009).Adenosine detection by using gold nanoparticles and designed aptamer sequences.ANALYST,134(7),1355.
MLA	Li, F,et al."Adenosine detection by using gold nanoparticles and designed aptamer sequences".ANALYST 134.7(2009):1355.