Isoforskolin pretreatment attenuates lipopolysaccharide-induced acute lung injury in animal models

CORC > 昆明植物研究所 > 中国科学院昆明植物研究所 > 植物化学与西部植物资源持续利用国家重点实验室

	Isoforskolin pretreatment attenuates lipopolysaccharide-induced acute lung injury in animal models
	Yang, Weimin 1; Qiang, Dongjin 1; Zhang, Min 1; Ma, Limei 1; Zhang, Yonghui 1; Qing, Chen 1; Xu, Yunlong 2; Zhen, Chunlan 1; Liu, Jikai 2; Chen, Yan-Hua 3
刊名	INTERNATIONAL IMMUNOPHARMACOLOGY
	2011-06-01
卷号	11 期号:6 页码:683-692
关键词	Acute lung injury Isoforskolin Lipopolysaccharide Inflammation Cytokine
ISSN号	1567-5769
通讯作者	Yang, WM (reprint author), Kunming Med Coll, Yunnan Key Lab Pharmacol Nat Prod, Kunming 650031, Peoples R China
英文摘要	Isoforskolin was isolated from Coleus forskohlii native to Yunnan in China. We hypothesize that isuforskolin pretreatment attenuates acute lung injury induced by lipopolysaccharide (endotoxin). Three acute lung injury models were used: situ perfused rat lung, rat and mouse models of endotoxic shock. Additionally, lipopolysaccharide stimulated proinflammatory cytokine production was evaluated in human mononuclear leukocyte. In situ perfused rat lungs, pre-perfusion with isoforskolin (100, and 200 mu M) and dexarrethasone (65 mu M, positive control) inhibited lipopolysaccharide (10 mg/L) induced increases in lung neutrophil adhesion rate, myeloperoxidase activity, lung weight Wet/Dry ratio, permeability-surface area product value, and tumor necrosis factor (TNF)-alpha levels. In rats, pretreatments with isoforskolin (5, 10, and 20 mg/kg, i.p.) and dexamethasone (5 mg/kg, i.p.) markedly reduced lipopolysaccharide (6 mg/kg i.v.) induced increases of karyocyte, neutrophil counts and protein content in bronchoalveolar lavage fluid, and plasma myeloperoxidase activity. Lung histopathology showed that morphologic changes induced by lipopolysaccharide were less pronounced in the isoforskolin and dexamethasone pretreated rats. In mice, 5 mg/kg isoforskolin and dexamethasone caused 100% and 80% survival, respectively, after administration of lipopolysaccharide (62.5 mg/kg, iv., 40% survival if untreated). In human mononuclear leukocyte, isoforskolin (50, 100, and 200 mu M) and dexamethasone (10 mu M) pre-incubation lowered lipopolysaccharide (2 mu g/mL) induced secretion of the cytokine TNF-alpha, and interleukins (IL)-1 beta, IL-6, and IL-8. In conclusion, pretreatment with isoforskolin attenuates lipopolysaccharide-induced acute lung injury in several models, and it is involved in down-regulation of inflammatory responses and proinflammatory cytokines TNF-alpha, IL-1 beta, IL-6, and IL-8. (C) 2011 Elsevier B.V. All rights reserved.
学科主题	Immunology; Pharmacology & Pharmacy
类目[WOS]	Immunology ; Pharmacology & Pharmacy
研究领域[WOS]	Immunology ; Pharmacology & Pharmacy
关键词[WOS]	ADENYLYL-CYCLASE ISOFORMS ; PROINFLAMMATORY CYTOKINE PRODUCTION ; RAT LUNGS ; ISCHEMIA-REPERFUSION ; EDEMA FORMATION ; ENDOTOXIN ; CAMP ; PENTOXIFYLLINE ; MACROPHAGES ; PERMEABILITY
收录类别	SCI
资助信息	National Natural Science Foundation of China[30560174]; Yunnan Provincial Natural Science Foundation[2004C0048M]
语种	英语
WOS记录号	WOS:000291505900007
公开日期	2012-04-10
内容类型	期刊论文
源URL	[http://ir.kib.ac.cn/handle/151853/5353]
专题	昆明植物研究所_植物化学与西部植物资源持续利用国家重点实验室
作者单位	1.Kunming Med Coll, Yunnan Key Lab Pharmacol Nat Prod, Kunming 650031, Peoples R China 2.Chinese Acad Sci, Kunming Inst Bot, State Key Lab Phytochem & Plant Resources W China, Kunming 650204, Peoples R China 3.E Carolina Univ, Brody Sch Med, Dept Anat & Cell Biol, Greenville, NC 27834 USA
推荐引用方式 GB/T 7714	Yang, Weimin,Qiang, Dongjin,Zhang, Min,et al. Isoforskolin pretreatment attenuates lipopolysaccharide-induced acute lung injury in animal models[J]. INTERNATIONAL IMMUNOPHARMACOLOGY,2011,11(6):683-692.
APA	Yang, Weimin.,Qiang, Dongjin.,Zhang, Min.,Ma, Limei.,Zhang, Yonghui.,...&Chen, Yan-Hua.(2011).Isoforskolin pretreatment attenuates lipopolysaccharide-induced acute lung injury in animal models.INTERNATIONAL IMMUNOPHARMACOLOGY,11(6),683-692.
MLA	Yang, Weimin,et al."Isoforskolin pretreatment attenuates lipopolysaccharide-induced acute lung injury in animal models".INTERNATIONAL IMMUNOPHARMACOLOGY 11.6(2011):683-692.