Mitochondria-dependent apoptosis of activated T lymphocytes induced by astin C, a plant cyclopeptide, for preventing murine experimental colitis

CORC > 昆明植物研究所 > 中国科学院昆明植物研究所 > 植物化学与西部植物资源持续利用国家重点实验室

	Mitochondria-dependent apoptosis of activated T lymphocytes induced by astin C, a plant cyclopeptide, for preventing murine experimental colitis
	Shen, Yan 2; Luo, Qiong 2; Xu, Huimin 1; Gong, Fangyuan 2; Zhou, Xiaobin 2; Sun, Yang 2; Wu, Xuefeng 2; Liu, Wen 2; Zeng, Guangzhi 1; Tan, Ninghua 1
刊名	BIOCHEMICAL PHARMACOLOGY
	2011-08-01
卷号	82 期号:3 页码:260-268
关键词	Astin C T lymphocyte Apoptosis Mitochondria Colitis
ISSN号	0006-2952
通讯作者	Tan, NH (reprint author), Chinese Acad Sci, State Key Lab Phytochem & Plant Resources W China, Kunming Inst Bot, Kunming 650204, Peoples R China
英文摘要	Facilitating T-cell apoptosis is implicated as an effective therapeutic strategy for treatment of T cell-mediated disease, including inflammatory bowel disease. Here, we report that astin C, a plant cyclopeptide isolated from the roots of Aster tataricus (Compositae), induced apoptosis of activated T cells in a mitochondria-dependent but Fas-independent manner in that such activity was still observed in T cells from Fas-mutated MRL(lpr/lpr) mice. Although caspase 8 was not activated, astin C treatment led to the cleavage of caspase 9 and caspase 3, the upregulation of Bad protein expression as well as release of cytochrome c in activated T cells. Astin C did not induce the expression of GRP78 and GADD153, excluding involvement of endoplasmic reticulum stress-mediated pathway. Moreover, oral administration of astin C protected mice against TNBS-induced colonic inflammation, as assessed by a reduced colonic weight/length ratio and histological scoring. Administering astin C significantly decreased serum levels of TNF-alpha, IL-4 and IL-17, accompanied with the induction of apoptosis in activated T cells in vivo. The results demonstrate, for the first time, the ability of astin C to induce apoptosis in activated T cells and its potential use in the treatment of colonic inflammation. (C) 2011 Elsevier Inc. All rights reserved.
学科主题	Pharmacology & Pharmacy
类目[WOS]	Pharmacology & Pharmacy
研究领域[WOS]	Pharmacology & Pharmacy
关键词[WOS]	INFLAMMATORY-BOWEL-DISEASE ; CROHNS-DISEASE ; CYCLIC PENTAPEPTIDES ; ASTER-TATARICUS ; IN-VIVO ; CELLS ; MICE ; INDUCTION ; RESISTANCE ; ANALOGS
收录类别	SCI
资助信息	National Natural Science Foundation of China[90913023, 30973920, 30973546]; Science Fund for Creative Research Groups[30821006]; Natural Science Foundation of Jiangsu Province[BK2008022, BK2009238]
语种	英语
WOS记录号	WOS:000292371400008
公开日期	2012-04-10
内容类型	期刊论文
源URL	[http://ir.kib.ac.cn/handle/151853/5315]
专题	昆明植物研究所_植物化学与西部植物资源持续利用国家重点实验室
作者单位	1.Chinese Acad Sci, State Key Lab Phytochem & Plant Resources W China, Kunming Inst Bot, Kunming 650204, Peoples R China 2.Nanjing Univ, State Key Lab Pharmaceut Biotechnol, Sch Life Sci, Nanjing 210093, Peoples R China
推荐引用方式 GB/T 7714	Shen, Yan,Luo, Qiong,Xu, Huimin,et al. Mitochondria-dependent apoptosis of activated T lymphocytes induced by astin C, a plant cyclopeptide, for preventing murine experimental colitis[J]. BIOCHEMICAL PHARMACOLOGY,2011,82(3):260-268.
APA	Shen, Yan.,Luo, Qiong.,Xu, Huimin.,Gong, Fangyuan.,Zhou, Xiaobin.,...&Xu, Qiang.(2011).Mitochondria-dependent apoptosis of activated T lymphocytes induced by astin C, a plant cyclopeptide, for preventing murine experimental colitis.BIOCHEMICAL PHARMACOLOGY,82(3),260-268.
MLA	Shen, Yan,et al."Mitochondria-dependent apoptosis of activated T lymphocytes induced by astin C, a plant cyclopeptide, for preventing murine experimental colitis".BIOCHEMICAL PHARMACOLOGY 82.3(2011):260-268.