Polycation-sensitive membrane electrode for determination of heparin based on controlled release of protamine

CORC > 烟台海岸带研究所 > 中国科学院烟台海岸带研究所 > 中科院海岸带环境过程与生态修复重点实验室 > 近岸生态与环境实验室

	Polycation-sensitive membrane electrode for determination of heparin based on controlled release of protamine
	Chen, Yan 1,2; Ding, Jiawang1 ; Qin, Wei1
刊名	ANALYST
	2012
卷号	137 期号:8 页码:1944-1949
关键词	POTENTIOMETRIC POLYANION SENSORS BIOANALYTICAL APPLICATIONS ELECTROCHEMICAL SENSOR SELECTIVE ELECTRODES RESPONSE MECHANISM POLYION CONTAMINANTS BINDING ASSAY
产权排序	[Chen, Yan; Ding, Jiawang; Qin, Wei] CAS, Key Lab Coastal Zone Environm Proc, Shandong Prov Key Lab Coastal Zone Environm Proc, Yantai Inst Coastal Zone Res YIC,YICCAS, Yantai 264003, Shandong, Peoples R China; [Chen, Yan] Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
中文摘要	A polycation-selective polymeric membrane electrode using dinonylnaphthalene sulfonate as an ion exchanger has been developed as a protamine controlled-release system for potentiometric detection of heparin. The incorporation of tetradodecylammonium tetrakis(4-chlorophenyl)borate as a lipophilic salt in the membrane dramatically improves the sensor's selectivity towards protamine over sodium ions via influencing the activity coefficient of protamine in the membrane, and a stable potential baseline can be obtained in the presence of an electrolyte background. The electrostatic binding interaction between heparin and protamine decreases the concentration of free protamine released at the sample-membrane interface and facilitates the stripping of protamine out of the membrane surface via the ion-exchange process with sodium ions, thus decreasing the membrane potential. Under optimal conditions, the proposed polymeric membrane electrode exhibits a linear relationship between the initial slope of the potential change and the heparin concentration in the range of 0.025-1.25 U mL(-1) with an improved detection limit of 0.01 U mL(-1).
英文摘要	A polycation-selective polymeric membrane electrode using dinonylnaphthalene sulfonate as an ion exchanger has been developed as a protamine controlled-release system for potentiometric detection of heparin. The incorporation of tetradodecylammonium tetrakis(4-chlorophenyl)borate as a lipophilic salt in the membrane dramatically improves the sensor's selectivity towards protamine over sodium ions via influencing the activity coefficient of protamine in the membrane, and a stable potential baseline can be obtained in the presence of an electrolyte background. The electrostatic binding interaction between heparin and protamine decreases the concentration of free protamine released at the sample-membrane interface and facilitates the stripping of protamine out of the membrane surface via the ion-exchange process with sodium ions, thus decreasing the membrane potential. Under optimal conditions, the proposed polymeric membrane electrode exhibits a linear relationship between the initial slope of the potential change and the heparin concentration in the range of 0.025-1.25 U mL(-1) with an improved detection limit of 0.01 U mL(-1).
学科主题	Chemistry
研究领域[WOS]	Chemistry
关键词[WOS]	POTENTIOMETRIC POLYANION SENSORS ; BIOANALYTICAL APPLICATIONS ; ELECTROCHEMICAL SENSOR ; SELECTIVE ELECTRODES ; RESPONSE MECHANISM ; POLYION ; CONTAMINANTS ; BINDING ; ASSAY
收录类别	SCI
资助信息	National Natural Science Foundation of China[41176081]; Outstanding Youth Natural Science Foundation of Shandong Province[JQ200814]; Taishan Scholar Program of Shandong Province[TS20081159]
语种	英语
WOS记录号	WOS:000301674600030
公开日期	2012-04-13
内容类型	期刊论文
源URL	[http://ir.yic.ac.cn/handle/133337/5412]
专题	烟台海岸带研究所_近岸生态与环境实验室
作者单位	1.CAS, Key Lab Coastal Zone Environm Proc, Shandong Prov Key Lab Coastal Zone Environm Proc, Yantai Inst Coastal Zone Res YIC,YICCAS, Yantai 264003, Shandong, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Chen, Yan,Ding, Jiawang,Qin, Wei. Polycation-sensitive membrane electrode for determination of heparin based on controlled release of protamine[J]. ANALYST,2012,137(8):1944-1949.
APA	Chen, Yan,Ding, Jiawang,&Qin, Wei.(2012).Polycation-sensitive membrane electrode for determination of heparin based on controlled release of protamine.ANALYST,137(8),1944-1949.
MLA	Chen, Yan,et al."Polycation-sensitive membrane electrode for determination of heparin based on controlled release of protamine".ANALYST 137.8(2012):1944-1949.