Isolation and characterization of 13 new microsatellite markers in the triangle mussel (Hyriopsis cumingii)

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	Isolation and characterization of 13 new microsatellite markers in the triangle mussel (Hyriopsis cumingii)
	Yuan, Han-wen 1; Guo, Long-gen 2; Xu, Qiao-qing 1
刊名	IRANIAN JOURNAL OF FISHERIES SCIENCES
	2011-10-01
卷号	10 期号:4 页码:631-640
关键词	Hyriopsis cumingii Microsatellite Polymorphism Genetic diversity
ISSN号	1562-2916
通讯作者	Xu, QQ (reprint author), Yangtze Univ, Engn Res Ctr Wetland Agr Middle Reaches Yangtze R, Minist Educ, Jinzhou 434025, Hubei Province, Peoples R China ; xuqiaoqing@163.com
中文摘要	Microsatellite enriched library of Hyriopsis cumingii was constructed according to the strong affinity between biotin and streptavidin. One hundred clones with foreign inserts were sequenced and 65 clones were screened for usable microsatellites, of which 36 were deemed unique, of sufficient length (more than 8 repeats) and possessed adequate flanking regions for primer designment. Among 36 primer pairs designed, 25 yielded scorable amplification products. Upon testing 30 individuals were sampled using the 25 pair primers from Dongting Lake of Hunan Province, China. Then thirteen polymorphic microsatellite markers were isolated and characterized. These loci exhibited high levels of genetic polymorphism, so the observed number of alleles per locus ranged from 4 to 9. The ranges of observed and expected heterozygosity were 0.2543 to 0.8913 and 0.3629 to 0.8217, respectively, and the average polymorphic information content was 0.5198. Two microsatellite loci were significantly deviated from Hardy-Weinberg equilibrium due to the presence of null alleles, and no linkage disequilibrium found. These microsatellite loci will be useful for assessing the genetic diversity and population structure of H cumingii.
英文摘要	Microsatellite enriched library of Hyriopsis cumingii was constructed according to the strong affinity between biotin and streptavidin. One hundred clones with foreign inserts were sequenced and 65 clones were screened for usable microsatellites, of which 36 were deemed unique, of sufficient length (more than 8 repeats) and possessed adequate flanking regions for primer designment. Among 36 primer pairs designed, 25 yielded scorable amplification products. Upon testing 30 individuals were sampled using the 25 pair primers from Dongting Lake of Hunan Province, China. Then thirteen polymorphic microsatellite markers were isolated and characterized. These loci exhibited high levels of genetic polymorphism, so the observed number of alleles per locus ranged from 4 to 9. The ranges of observed and expected heterozygosity were 0.2543 to 0.8913 and 0.3629 to 0.8217, respectively, and the average polymorphic information content was 0.5198. Two microsatellite loci were significantly deviated from Hardy-Weinberg equilibrium due to the presence of null alleles, and no linkage disequilibrium found. These microsatellite loci will be useful for assessing the genetic diversity and population structure of H cumingii.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Fisheries
研究领域[WOS]	Fisheries
关键词[WOS]	WATER PEARL MUSSEL ; BIVALVIA ; LOCI ; DNA
收录类别	SCI
资助信息	Yangtze University, Jingzhou, Hubei Province, China[801200010112]
语种	英语
WOS记录号	WOS:000296392900008
公开日期	2012-04-01
内容类型	期刊论文
源URL	[http://ir.ihb.ac.cn/handle/342005/16729]
专题	水生生物研究所_淡水生态学研究中心_期刊论文
作者单位	1.Yangtze Univ, Engn Res Ctr Wetland Agr Middle Reaches Yangtze R, Minist Educ, Jinzhou 434025, Hubei Province, Peoples R China 2.Chinese Acad Sci, Donghu Expt Stn Lake Ecosyst, State Key Lab Freshwater Ecol & Biotechnol China, Inst Hydrobiol, Wuhan 430072, Peoples R China
推荐引用方式 GB/T 7714	Yuan, Han-wen,Guo, Long-gen,Xu, Qiao-qing. Isolation and characterization of 13 new microsatellite markers in the triangle mussel (Hyriopsis cumingii)[J]. IRANIAN JOURNAL OF FISHERIES SCIENCES,2011,10(4):631-640.
APA	Yuan, Han-wen,Guo, Long-gen,&Xu, Qiao-qing.(2011).Isolation and characterization of 13 new microsatellite markers in the triangle mussel (Hyriopsis cumingii).IRANIAN JOURNAL OF FISHERIES SCIENCES,10(4),631-640.
MLA	Yuan, Han-wen,et al."Isolation and characterization of 13 new microsatellite markers in the triangle mussel (Hyriopsis cumingii)".IRANIAN JOURNAL OF FISHERIES SCIENCES 10.4(2011):631-640.