Optimal strategies for determination of free/extractable and total microcystins in lake sediment

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	Optimal strategies for determination of free/extractable and total microcystins in lake sediment
	Wu, Xingqiang 2; Wang, Chunbo 1,3; Xiao, Bangding 1; Wang, Yang 2; Zheng, Na 2; Liu, Jingshuang 2
刊名	ANALYTICA CHIMICA ACTA
	2012-01-04
卷号	709 期号:0 页码:66-72
关键词	Microcystin 2-Methyl-3-methoxy-4-phenylbutyric acid (MMPB) Sediment Solvent extraction Lemieux oxidation
ISSN号	0003-2670
通讯作者	Xiao, BD (reprint author), Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China
中文摘要	The optimization of analytical procedures for the quantification of free and total microcystins (MCs) in natural sediments was systematically examined based on solvent extraction and Lemieux oxidation. In this optimized analytical procedure, a sequential solvent extraction using 50% (v/v) methanol and EDTA-sodium pyrophosphate was selected as the optimal extraction solvent for free MCs analysis, after which the purified extracts and sediment residuals were applied to the optimized Lemieux oxidation for determination of total MCs in lake sediments. The optimized procedures were shown to be efficient and reliable for the routine analysis of both free and total MCs in lake sediment samples, as indicated by the minimal adverse impact of sediment organic matter on the recovery of free MCs and yield of MMPB (2-methyl-3-methoxy-4-phenylbutyric acid). Finally, the developed procedures were applied to field sediment samples collected from Lake Dianchi during a bloom season and seven of thirty samples showed positive results. (C) 2011 Elsevier B.V. All rights reserved.
英文摘要	The optimization of analytical procedures for the quantification of free and total microcystins (MCs) in natural sediments was systematically examined based on solvent extraction and Lemieux oxidation. In this optimized analytical procedure, a sequential solvent extraction using 50% (v/v) methanol and EDTA-sodium pyrophosphate was selected as the optimal extraction solvent for free MCs analysis, after which the purified extracts and sediment residuals were applied to the optimized Lemieux oxidation for determination of total MCs in lake sediments. The optimized procedures were shown to be efficient and reliable for the routine analysis of both free and total MCs in lake sediment samples, as indicated by the minimal adverse impact of sediment organic matter on the recovery of free MCs and yield of MMPB (2-methyl-3-methoxy-4-phenylbutyric acid). Finally, the developed procedures were applied to field sediment samples collected from Lake Dianchi during a bloom season and seven of thirty samples showed positive results. (C) 2011 Elsevier B.V. All rights reserved.
WOS标题词	Science & Technology ; Physical Sciences
类目[WOS]	Chemistry, Analytical
研究领域[WOS]	Chemistry
关键词[WOS]	COVALENTLY BOUND MICROCYSTINS ; LIQUID-CHROMATOGRAPHY ; RISK-ASSESSMENT ; ANIMAL-TISSUES ; LR ; WATER ; CYANOBACTERIA ; EXTRACTION ; BIOACCUMULATION ; OPTIMIZATION
收录类别	SCI
资助信息	National Natural Science Foundation of China[41001326]; National Key Project for Basic Research[2008CB418101]; Chinese Academy of Sciences[KZCX2-YW-QN306]
语种	英语
WOS记录号	WOS:000299801500008
公开日期	2012-04-01
内容类型	期刊论文
源URL	[http://ir.ihb.ac.cn/handle/342005/16749]
专题	水生生物研究所_水环境工程研究中心_期刊论文
作者单位	1.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China 2.Chinese Acad Sci, NE Inst Geog & Agroecol, Key Lab Wetland Ecol & Environm, Changchun 130012, Peoples R China 3.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
推荐引用方式 GB/T 7714	Wu, Xingqiang,Wang, Chunbo,Xiao, Bangding,et al. Optimal strategies for determination of free/extractable and total microcystins in lake sediment[J]. ANALYTICA CHIMICA ACTA,2012,709(0):66-72.
APA	Wu, Xingqiang,Wang, Chunbo,Xiao, Bangding,Wang, Yang,Zheng, Na,&Liu, Jingshuang.(2012).Optimal strategies for determination of free/extractable and total microcystins in lake sediment.ANALYTICA CHIMICA ACTA,709(0),66-72.
MLA	Wu, Xingqiang,et al."Optimal strategies for determination of free/extractable and total microcystins in lake sediment".ANALYTICA CHIMICA ACTA 709.0(2012):66-72.