Detection of alpha-fetoprotein through biological signal amplification by biosensor based on imaging ellipsometry

	Detection of alpha-fetoprotein through biological signal amplification by biosensor based on imaging ellipsometry
	Wang CX; Jin G(靳刚); Chen YY(陈艳艳)
	2011
会议名称	5th International Conference on Spectroscopic Ellipsometry
会议日期	MAY 23-29, 2010
通讯作者邮箱	yychen2006@sinano.ac.cn; gajin@imech.ac.cn
会议地点	Albany, NY
关键词	Imaging ellipsometry Biosensor Signal amplification Alpha-fetoprotein Hepatocellular carcinoma PROTEIN MICROARRAYS VISUALIZATION PROSPECTS CANCER SERUM
卷号	519
期号	9
页码	2763-2767
通讯作者	Chen, YY (reprint author), Chinese Acad Sci, Suzhou Inst Nanotech & Nanobion, Suzhou, Peoples R China
中文摘要	High sensitive and quantitative detection of alpha-fetoprotein (AFP) by biosensor based on imaging ellipsometry (BIE) through biological amplification was investigated. AFP firstly reacted with the rat monoclonal antibody (rat-mAb) initially immobilized on glutaraldehyde modified silicon surface, then rabbit anti-human AFP polyclonal antibodies (Rabbit-pAb) and goat anti-rabbit IgG (goat-IgG) were sequentially applied to amplify signal. Results revealed that signal was enhanced approximately six fold. The linear range of AFP detection was 20.0-200.0 ng/ml with a low limit of 5.0 ng/ml (S/N = 3). The cross-reaction rate was less than 5.2% evaluated by biomarker (carcinoembryonic antigen, carbohydrate 19-9 and carbohydrate antigen 242) and two common proteins (human serum albumin, fibrinogen) and their mixture. Coefficient variation (CV) for intra-slide and inter-slide reproducibility were 10.3%, 6.6%, 6.3% and 10.7%, 7.9%, 6.4% for 41.6 ng/ml, 83.2 ng/ml and 128.4 ng/ml AFP. In clinic application, cut-off value at 28.6 ng/ml was also determined with sensitivity 0.72 and specificity 0.94. Results of 47 clinic patient samples detected by BIE were in good agreement with those of electrochemiluminescence immunoassay (ECLIA) (R2 = 0.9949). The area under receiver operating characteristic curve (ROC) curve is 0.89. Statistical analysis showed that BIE is high agreement with ECLIA (Kappa = 0.733, U = 3.21>U0.01). It shows a potential for hepatocellular carcinoma (HCC) diagnosis. (C) 2010 Elsevier B.V. All rights reserved.
收录类别	CPCI（ISTP）
合作状况	国内
产权排序	Chinese Acad Sci, Suzhou Inst Nanotech & Nanobion, Suzhou, Peoples R China; Chinese Acad Sci, Inst Mech, NML, Beijing 100080, Peoples R China; Chinese Acad Sci, Inst Biophys, Beijing 100080, Peoples R China; Chinese Acad Sci, Grad Sch, Beijing, Peoples R China; Shandong Acad Med Sci, Radiat Med Inst, Jinan, Peoples R China
会议主办者	Univ Albany, Coll Nanoscale & Engn
会议网址	http://dx.doi.org/10.1016/j.tsf.2010.11.064
会议录	THIN SOLID FILMS
会议录出版者	ELSEVIER SCIENCE SA
会议录出版地	PO BOX 564, 1001 LAUSANNE, SWITZERLAND
学科主题	Materials Science, Multidisciplinary; Materials Science, Coatings & Films; Physics, Applied; Physics, Condensed Matter
语种	英语
ISSN号	0040-6090
内容类型	会议论文
源URL	[http://dspace.imech.ac.cn/handle/311007/45316]
专题	力学研究所_国家微重力实验室
推荐引用方式 GB/T 7714	Wang CX,Jin G,Chen YY. Detection of alpha-fetoprotein through biological signal amplification by biosensor based on imaging ellipsometry[C]. 见:5th International Conference on Spectroscopic Ellipsometry. Albany, NY. MAY 23-29, 2010.http://dx.doi.org/10.1016/j.tsf.2010.11.064.