Gene expression and function study of fusion immunotoxin anti-Her-2-scFv - SEC2 in Escherichia coli

CORC > 沈阳应用生态研究所 > 中国科学院沈阳应用生态研究所 > 沈阳应用生态研究所 > 期刊论文

	Gene expression and function study of fusion immunotoxin anti-Her-2-scFv - SEC2 in Escherichia coli
	Xu, MK ; Zhang, CG
刊名	APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
	2006
卷号	70 期号:1 页码:78-84
ISSN号	0175-7598
通讯作者	Xu, MK, 72 Wenhua Rd,POB 417, Shenyang 110016, Peoples R China
中文摘要	The whole sec2 DNA fragment was obtained by polymerase chain reaction with total genomic DNA extracted from Staphylococcus aureus. The newly discovered gene contains 717 bp (GenBank Accession number AY450554) and encodes 239 amino acids in accordance with the protein sequence reported in GenBank. The sec2 gene was fused to anti-Her-2 scFv gene with a DNA linker at the upstream to construct the fusion gene of immunotoxin which was subcloned to expression vector pET-32a(+) and expressed efficiently in Escherichia coli. The purified fusion immunotoxin could target the HER-2 overexpressed by breast tumor cell SK-Br-3 in vitro and demonstrate tumor-inhibition effect on SK-Br-3.
学科主题	Biotechnology & Applied Microbiology
收录类别	SCI
语种	英语
WOS记录号	WOS:000235306800012
公开日期	2011-09-23
内容类型	期刊论文
源URL	[http://210.72.129.5/handle/321005/55011]
专题	沈阳应用生态研究所_沈阳应用生态研究所_期刊论文
推荐引用方式 GB/T 7714	Xu, MK,Zhang, CG. Gene expression and function study of fusion immunotoxin anti-Her-2-scFv - SEC2 in Escherichia coli[J]. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,2006,70(1):78-84.
APA	Xu, MK,&Zhang, CG.(2006).Gene expression and function study of fusion immunotoxin anti-Her-2-scFv - SEC2 in Escherichia coli.APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,70(1),78-84.
MLA	Xu, MK,et al."Gene expression and function study of fusion immunotoxin anti-Her-2-scFv - SEC2 in Escherichia coli".APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 70.1(2006):78-84.