Simultaneous determination of amino acids in soil by reversed phase high performance liquid chromatography by using 6-aminoquinoly-N-hydroxysuccinimidyl carbamate as a precolumn derivatization reagent

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	Simultaneous determination of amino acids in soil by reversed phase high performance liquid chromatography by using 6-aminoquinoly-N-hydroxysuccinimidyl carbamate as a precolumn derivatization reagent
	Hou, SM ; Sun, J ; He, HB ; Zhang, XD ; Wang, YH
刊名	CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
	2006
卷号	34 期号:10 页码:1395-1400
关键词	reversed phase high performance liquid chromatography pre-column derivatization 6-aminoquinoly-N-hydroxysuccinimidyl carbamate amino acids soil mass spectrometric
ISSN号	0253-3820
通讯作者	Zhang, XD, Chinese Acad Sci, Inst Appl Ecol, Key Lab Terr Ecol Proc, Shenyang 110016, Peoples R China
中文摘要	A reversed phase high performance liquid chromatographic method for simultaneous determination of 17 amino acids in soil was established. The amino acid samples were mixed with 2-aminobutyric acid as the internal standard, derivatized with 6-aminoquinolyl-N-hydroxysuceinimidyl carbarnate (AQC), and then separated on a XTerra C(18)column using 1.8 mmol/L formate ammonium (pH = 3. 0) and acetonitrile solution as a mobile phase with gradient elution technique. The photodiode detector at 248 nm was utilized for amino acid quantification. All 17 amino acid derivatives were separated well when an optimized elution condition was applied. Consequently, good reproducibility, high sensitivity and high precision of the method were achieved. When the amino acid concentration was set at the range of 25 - 600 mu mol/L, the linear correlation coefficients of the amino acids were above 0.99 and the detector limit of each amino acids was lower than 0.5 pmol. Total ion current chromatograms of mass spectrum of 17 AQC derivatized amino acids were obtained. The method provids a new way for intensifying the study of amino acids in soil and environment.
学科主题	Chemistry, Analytical
收录类别	SCI
语种	中文
WOS记录号	WOS:000243502600008
公开日期	2011-09-23
内容类型	期刊论文
源URL	[http://210.72.129.5/handle/321005/54777]
专题	沈阳应用生态研究所_沈阳应用生态研究所_期刊论文
推荐引用方式 GB/T 7714	Hou, SM,Sun, J,He, HB,et al. Simultaneous determination of amino acids in soil by reversed phase high performance liquid chromatography by using 6-aminoquinoly-N-hydroxysuccinimidyl carbamate as a precolumn derivatization reagent[J]. CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,2006,34(10):1395-1400.
APA	Hou, SM,Sun, J,He, HB,Zhang, XD,&Wang, YH.(2006).Simultaneous determination of amino acids in soil by reversed phase high performance liquid chromatography by using 6-aminoquinoly-N-hydroxysuccinimidyl carbamate as a precolumn derivatization reagent.CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,34(10),1395-1400.
MLA	Hou, SM,et al."Simultaneous determination of amino acids in soil by reversed phase high performance liquid chromatography by using 6-aminoquinoly-N-hydroxysuccinimidyl carbamate as a precolumn derivatization reagent".CHINESE JOURNAL OF ANALYTICAL CHEMISTRY 34.10(2006):1395-1400.