Impact assessment of cadmium contamination on rice (Oryza sativa L.) seedlings at molecular and population levels using multiple biomarkers

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	Impact assessment of cadmium contamination on rice (Oryza sativa L.) seedlings at molecular and population levels using multiple biomarkers
	Liu, W ; Yang, YS ; Zhou, Q ; Xie, L ; Li, P ; Sun, T
刊名	CHEMOSPHERE
	2007
卷号	67 期号:6 页码:1155-1163
关键词	cadmium (Cd) rice root growth total soluble protein level genomic template stability biomarker random amplified polymorphic DNA (RAPD)
ISSN号	0045-6535
通讯作者	Yang, YS, Cardiff Univ, Sch Earth Ocean & Planetary Sci, Cardiff CF10 3YE, Wales
中文摘要	Assessment of environmental contamination on ecology (plant) at molecular and population levels is important in risk quantification and remediation study. Random amplified polymorphic DNA (RAPD) assay and related other fingerprinting techniques have been employed to detect the genotoxin-induced DNA damage and mutations. This research compared the effects occurring at molecular and population levels in rice seedlings exposed to cadmium (Cd) concentrations of 15-60 mg l(-1) for 8 days with quartz sand culture. Inhibition of root growth and increase of total soluble protein content in root tips of rice seedlings were observed with the increase of Cd concentration. For the RAPD analyses, 12 RAPD primers of 50-70% GC content were found to produce unique polymorphic band patterns and subsequently were used to produce a total of 180 bands of 179-3056 bp in molecular size. in the control root tips of rice seedlings. Results produced by these RAPD primers indicate that changes in RAPD profiles of root tips after Cd treatment include modifications in band intensity and gain or loss of bands by comparison with control. The effect of changes was dose-dependent. Genomic template stability compares favourably with the traditional indices such as root growth and soluble protein content. The DNA polymorphisms detected by RAPD analysis can be applied as a suitable biomarker assay for the detection of genotoxic effects of Cd contamination on plants. (c) 2006 Elsevier Ltd. All rights reserved.
学科主题	Environmental Sciences
收录类别	SCI
语种	英语
WOS记录号	WOS:000245846700013
公开日期	2011-09-23
内容类型	期刊论文
源URL	[http://210.72.129.5/handle/321005/54723]
专题	沈阳应用生态研究所_沈阳应用生态研究所_期刊论文
推荐引用方式 GB/T 7714	Liu, W,Yang, YS,Zhou, Q,et al. Impact assessment of cadmium contamination on rice (Oryza sativa L.) seedlings at molecular and population levels using multiple biomarkers[J]. CHEMOSPHERE,2007,67(6):1155-1163.
APA	Liu, W,Yang, YS,Zhou, Q,Xie, L,Li, P,&Sun, T.(2007).Impact assessment of cadmium contamination on rice (Oryza sativa L.) seedlings at molecular and population levels using multiple biomarkers.CHEMOSPHERE,67(6),1155-1163.
MLA	Liu, W,et al."Impact assessment of cadmium contamination on rice (Oryza sativa L.) seedlings at molecular and population levels using multiple biomarkers".CHEMOSPHERE 67.6(2007):1155-1163.