Toxic effects of acetochlor, methamidophos and their combination on nifH gene in soil

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	Toxic effects of acetochlor, methamidophos and their combination on nifH gene in soil
	Su, ZC ; Zhang, HW ; Li, XY ; Zhang, Q ; Zhang, CG
刊名	JOURNAL OF ENVIRONMENTAL SCIENCES-CHINA
	2007
卷号	19 期号:7 页码:864-873
关键词	agrochemicals acetochlor methamidophos toxic effects nifH gene PCR-DGGE
ISSN号	1001-0742
通讯作者	Zhang, HW, Chinese Acad Sci, Inst Appl Ecol, Key Lab Terr Ecol Proc, Shenyang 110016, Peoples R China
中文摘要	Toxic effects of two agrochemicals on nifH gene in agricultural black soil were investigated using denaturing gradient gel electrophoresis (DGGE) and sequencing approaches in a microcosm experiment. Changes of soil nifH gene diversity and composition were examined following the application of acetochlor, methamidophos and their combination. Acetochlor reduced the nifH gene diversity (both in gene richness and diversity index values) and caused changes in the nifH gene composition. The effects of acetochlor on nifH gene were strengthened as the concentration of acetochlor increased. Cluster analysis of DGGE banding patterns showed that nifH gene composition which had been affected by low concentration of acetochlor (50 mg/kg) recovered firstly. Methamidophos reduced nifH gene richness that except at 4 weeks. The medium concentration of methamidophos (150 mg/kg) caused the most apparent changes in nifH gene diversity at the first week while the high concentration of methamidophos (250 mg/kg) produced prominent effects on nifH gene diversity in the following weeks. Cluster analysis showed that minimal changes of nifH gene composition were found at 1 week and maximal changes at 4 weeks. Toxic effects of acetochlor and methamidophos combination on nifH gene were also apparent. Different nifH genes (bands) responded differently to the impact of agrochemicals: four individual bands were eliminated by the application of the agrochemicals, five bands became predominant by the stimulation of the agrochemicals, and four bands showed strong resistance to the influence of the agrochemicals. Fifteen prominent bands were partially sequenced, yielding 15 different nifH sequences, which were used for phylogenetic reconstructions. All sequences were affiliated with the alpha- and beta-proteobacteria, showing higher similarity to eight different diazotrophic genera.
学科主题	Environmental Sciences
收录类别	SCI
语种	英语
WOS记录号	WOS:000248095400016
公开日期	2011-09-23
内容类型	期刊论文
源URL	[http://210.72.129.5/handle/321005/54669]
专题	沈阳应用生态研究所_沈阳应用生态研究所_期刊论文
推荐引用方式 GB/T 7714	Su, ZC,Zhang, HW,Li, XY,et al. Toxic effects of acetochlor, methamidophos and their combination on nifH gene in soil[J]. JOURNAL OF ENVIRONMENTAL SCIENCES-CHINA,2007,19(7):864-873.
APA	Su, ZC,Zhang, HW,Li, XY,Zhang, Q,&Zhang, CG.(2007).Toxic effects of acetochlor, methamidophos and their combination on nifH gene in soil.JOURNAL OF ENVIRONMENTAL SCIENCES-CHINA,19(7),864-873.
MLA	Su, ZC,et al."Toxic effects of acetochlor, methamidophos and their combination on nifH gene in soil".JOURNAL OF ENVIRONMENTAL SCIENCES-CHINA 19.7(2007):864-873.