Functional analysis of the disulphide loop mutant of staphylococcal enterotoxin C2

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	Functional analysis of the disulphide loop mutant of staphylococcal enterotoxin C2
	Wang, XG ; Xu, MK ; Cai, YM ; Yang, HL ; Zhang, HW ; Zhang, CG
刊名	APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
	2009
卷号	82 期号:5 页码:861-871
关键词	Superantigen Staphylococcal enterotoxin C2 Immunotherapy Toxicity The disulphide loop Site-directed mutagenesis
ISSN号	0175-7598
通讯作者	Xu, MK, Chinese Acad Sci, Inst Appl Ecol, Shenyang 110016, Peoples R China
中文摘要	The superantigen staphylococcal enterotoxin C2 (SEC2) tremendously activate T lymphocytes bearing certain T-cell receptor V beta domains when binding to MHC II molecules, which launches a powerful response of tumour inhibition in vitro as well as in vivo. However, the toxicity of SEC2 performed in clinic limited its broad application for immunotherapy. The previous studies suggested that the disulphide loop may be important for the toxicity of some SEs, which prompted us to investigate the potential roles of the disulphide loop in biological activity of SEC2. Site-directed mutagenesis was used to disturb the formation of the disulphide bond by substituting Ala or Ser for Cys-93 and Cys-110. The expressed mutants in Escherichia coli were used to determine their superantigen activity and toxicity. Results showed that all of the mutated proteins exhibited reduced abilities to induce T-cell proliferation and cytotoxic effects on tumour cells L929 and Hepa1-6, suggesting that the disulphide loop plays functional role in maintaining the maximal superantigen activity of SEC2. Furthermore, the toxicity assays in vivo showed that all of the mutants induced a reduced emetic and pyrogenic responses compared with native SEC2, which might be important for further construction of lowly toxic superantigen agent.
学科主题	Biotechnology & Applied Microbiology
收录类别	SCI
资助信息	Institute of Applied Ecology of the CAS [08SBS111S3]; Shenyang Xiehe Bio-pharmaceutical Co. Ltd
语种	英语
WOS记录号	WOS:000264460500008
公开日期	2011-09-23
内容类型	期刊论文
源URL	[http://210.72.129.5/handle/321005/54203]
专题	沈阳应用生态研究所_沈阳应用生态研究所_期刊论文
推荐引用方式 GB/T 7714	Wang, XG,Xu, MK,Cai, YM,et al. Functional analysis of the disulphide loop mutant of staphylococcal enterotoxin C2[J]. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,2009,82(5):861-871.
APA	Wang, XG,Xu, MK,Cai, YM,Yang, HL,Zhang, HW,&Zhang, CG.(2009).Functional analysis of the disulphide loop mutant of staphylococcal enterotoxin C2.APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,82(5),861-871.
MLA	Wang, XG,et al."Functional analysis of the disulphide loop mutant of staphylococcal enterotoxin C2".APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 82.5(2009):861-871.