Characterization of the tandem-arrayed hiC6 genes in Antarctic and temperate strains of Chlorella vulgaris

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	Characterization of the tandem-arrayed hiC6 genes in Antarctic and temperate strains of Chlorella vulgaris
	Wang, Yali; Liu, Xiaoxiang; Gao, Hong; Xu, Xudong
刊名	FEMS MICROBIOLOGY LETTERS
	2011-12-01
卷号	325 期号:2 页码:130-139
关键词	Chlorella vulgaris Antarctic strain temperate strain hiC6 tandem array
ISSN号	0378-1097
通讯作者	Xu, XD (reprint author), Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Hunan, Peoples R China ; xux@ihb.ac.cn
中文摘要	HIC6 is a group-3 late embryogenesis abundant protein found in Chlorella vulgaris. In the Antarctic strain NJ-7 of this unicellular green alga, it is encoded by a tandem array of five hiC6 genes (designated as NJ7hiC6-1, -2, -3, -4 and -5); in the temperate strain UTEX259, it is encoded by four hiC6 genes in tandem (designated as 259hiC6-1, -2, -3 and -4). Except for NJ7hiC6-3 and -4, the encoding regions of all other hiC6 genes differ from each other by 219 bp in each strain. Based on RT-PCR and sequencing of total hiC6 cDNA clones, the relative transcript abundance of each hiC6 gene was evaluated. NJ7hiC6-2 and 259hiC6-2 were not expressed or expressed at low levels, whereas 259hiC6-1 and NJ7hiC6-3/4 exhibited the highest hiC6 transcript levels in the respective strains. In vitro assays showed that different isoforms of HIC6 provided almost identical cryoprotection of lactate dehydrogenase. Our studies suggest that the formation of the tandem arrays of hiC6 in Chlorella is a process of gene duplications accompanied by gene expression divergence.
英文摘要	HIC6 is a group-3 late embryogenesis abundant protein found in Chlorella vulgaris. In the Antarctic strain NJ-7 of this unicellular green alga, it is encoded by a tandem array of five hiC6 genes (designated as NJ7hiC6-1, -2, -3, -4 and -5); in the temperate strain UTEX259, it is encoded by four hiC6 genes in tandem (designated as 259hiC6-1, -2, -3 and -4). Except for NJ7hiC6-3 and -4, the encoding regions of all other hiC6 genes differ from each other by 219 bp in each strain. Based on RT-PCR and sequencing of total hiC6 cDNA clones, the relative transcript abundance of each hiC6 gene was evaluated. NJ7hiC6-2 and 259hiC6-2 were not expressed or expressed at low levels, whereas 259hiC6-1 and NJ7hiC6-3/4 exhibited the highest hiC6 transcript levels in the respective strains. In vitro assays showed that different isoforms of HIC6 provided almost identical cryoprotection of lactate dehydrogenase. Our studies suggest that the formation of the tandem arrays of hiC6 in Chlorella is a process of gene duplications accompanied by gene expression divergence.
学科主题	Microbiology
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Microbiology
研究领域[WOS]	Microbiology
关键词[WOS]	EMBRYOGENESIS ABUNDANT PROTEINS ; HARDENING-INDUCED GENES ; FREEZING TOLERANCE ; LEA PROTEINS ; IDENTIFICATION ; EXPRESSION ; C-27 ; ELLIPSOIDEA ; DEHYDRINS ; ENCODES
收录类别	SCI
资助信息	Chinese Academy of Sciences[KSCX2-YW-G-060, KSCX2-SW-332]
语种	英语
WOS记录号	WOS:000297208600005
公开日期	2011-12-22
内容类型	期刊论文
源URL	[http://ir.ihb.ac.cn/handle/342005/16475]
专题	水生生物研究所_藻类生物学及应用研究中心_期刊论文
作者单位	Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Hunan, Peoples R China
推荐引用方式 GB/T 7714	Wang, Yali,Liu, Xiaoxiang,Gao, Hong,et al. Characterization of the tandem-arrayed hiC6 genes in Antarctic and temperate strains of Chlorella vulgaris[J]. FEMS MICROBIOLOGY LETTERS,2011,325(2):130-139.
APA	Wang, Yali,Liu, Xiaoxiang,Gao, Hong,&Xu, Xudong.(2011).Characterization of the tandem-arrayed hiC6 genes in Antarctic and temperate strains of Chlorella vulgaris.FEMS MICROBIOLOGY LETTERS,325(2),130-139.
MLA	Wang, Yali,et al."Characterization of the tandem-arrayed hiC6 genes in Antarctic and temperate strains of Chlorella vulgaris".FEMS MICROBIOLOGY LETTERS 325.2(2011):130-139.