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	Amplified voltammetric detection of glycoproteins using 4-mercaptophenylboronic acid/biotin-modified multifunctional gold nanoparticles as labels
	Liu, Lin*; Xing, Yun; Zhang, Hui; Liu, Ruili; Liu, Huijing; Xia, Ning
刊名	International Journal of Nanomedicine
	2014
卷号	9期号:1页码:2619-2626
关键词	Ultrasensitive detection of protein biomarkers is essential for early diagnosis and therapy of many diseases. Glycoproteins differing from other types of proteins contain carbohydrate moieties in the oligosaccharide chains. Boronic acid can form boronate ester covalent bonds with diol-containing species. Herein we present a sensitive and cost-effective electrochemical method for glycoprotein detection using 4-mercaptophenylboronic acid (MBA)/biotin-modified gold nanoparticles (AuNPs) (MBA-biotin-AuNPs) as labels. To demonstrate the feasibility and sensitivity of this method recombinant human erythropoietin (rHuEPO) was tested as a model analyte. Specifically rHuEPO was captured by the anti-rHuEPO aptamer-covered electrode and then derivatized with MBA-biotin-AuNPs through the boronic acid–carbohydrate interaction. The MBA-biotin-AuNPs facilitated the attachment of streptavidin-conjugated alkaline phosphatase for the production of electroactive p-aminophenol from p-aminophenyl phosphate substrate. A detection limit of 8 fmol L-1 for rHuEPO detection was achieved. Other glycosylated and non-glycosylated proteins such as horseradish peroxidase prostate specific antigen metallothionein streptavidin and thrombin showed no interference in the detection assay.
ISSN号	1178-2013
DOI	10.2147/IJN.S62343
URL标识	查看原文
WOS记录号	WOS:000336442600001
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/3334629
专题	中南大学
作者单位	1.[Zhang, Hui 2.Xia, Ning 3.Liu, Ruili 4.Xing, Yun 5.Liu, Huijing 6.Liu, Lin] Anyang Normal Univ, Coll Chem & Chem Engn, Anyang 455000, Henan, Peoples R China.
推荐引用方式 GB/T 7714	Liu, Lin*,Xing, Yun,Zhang, Hui,et al. Amplified voltammetric detection of glycoproteins using 4-mercaptophenylboronic acid/biotin-modified multifunctional gold nanoparticles as labels[J]. International Journal of Nanomedicine,2014,9(1):2619-2626.
APA	Liu, Lin*,Xing, Yun,Zhang, Hui,Liu, Ruili,Liu, Huijing,&Xia, Ning.(2014).Amplified voltammetric detection of glycoproteins using 4-mercaptophenylboronic acid/biotin-modified multifunctional gold nanoparticles as labels.International Journal of Nanomedicine,9(1),2619-2626.
MLA	Liu, Lin*,et al."Amplified voltammetric detection of glycoproteins using 4-mercaptophenylboronic acid/biotin-modified multifunctional gold nanoparticles as labels".International Journal of Nanomedicine 9.1(2014):2619-2626.

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