Characterization of a Selenium-resistance-enhancing Homocysteine S-methyltransferase from Aegilops tauschii

	Characterization of a Selenium-resistance-enhancing Homocysteine S-methyltransferase from Aegilops tauschii
	Wu, L. J.; Shang, Y.; Liu, T.; Chen, W. J.; Liu, B. L.; Zhang, L. Q.; Liu, D. C.; Zhang, B.; Zhang, H. G.
刊名	CEREAL RESEARCH COMMUNICATIONS
	2018
卷号	46 期号:2
关键词	homocysteine S-methyltransferase Aegilops tauschii selenium
英文摘要	In this study, the cDNA of homocysteine S-methyltransferase was isolated from Aegilops tauschii Coss., with the gene accordingly designated as AetHMT1. Similar to other methyl-transferases, AetHMT1 contains a GGCCR consensus sequence for a possible zinc-binding motif near the C-terminal and a conserved cysteine residue upstream of the zinc-binding motif. Analysis of AetHMT1 uncovered no obvious chloroplast or mitochondrial targeting sequences. We functionally expressed AetHMT1 in Escherichia coli and confirmed its biological activity, as evidenced by a positive HMT enzyme activity of 164.516 +/- 17.378 nmol min(-1) mg(-1) protein when catalyzing the transformation of L-homocysteine. Compared with the bacterium containing the empty vector, E. coli harboring the recombinant AetHMT1 plasmid showed much higher tolerance to selenate and selenite. AetHMT1 transcript amounts in different organs were increased by Na2SeO4 treatment, with roots accumulating higher amounts than stems, old leaves and new leaves. We have therefore successfully isolated HMT1 from Ae. tauschii and characterized the biochemical and physiological functions of the corresponding protein.
内容类型	期刊论文
源URL	[http://210.75.249.4/handle/363003/60011]
专题	西北高原生物研究所_中国科学院西北高原生物研究所
推荐引用方式 GB/T 7714	Wu, L. J.,Shang, Y.,Liu, T.,et al. Characterization of a Selenium-resistance-enhancing Homocysteine S-methyltransferase from Aegilops tauschii[J]. CEREAL RESEARCH COMMUNICATIONS,2018,46(2).
APA	Wu, L. J..,Shang, Y..,Liu, T..,Chen, W. J..,Liu, B. L..,...&Zhang, H. G..(2018).Characterization of a Selenium-resistance-enhancing Homocysteine S-methyltransferase from Aegilops tauschii.CEREAL RESEARCH COMMUNICATIONS,46(2).
MLA	Wu, L. J.,et al."Characterization of a Selenium-resistance-enhancing Homocysteine S-methyltransferase from Aegilops tauschii".CEREAL RESEARCH COMMUNICATIONS 46.2(2018).