Emissions of terbium metal-organic frameworks modulated by dispersive/agglomerated gold nanoparticles for the construction of prostate-specific antigen biosensor

	Emissions of terbium metal-organic frameworks modulated by dispersive/agglomerated gold nanoparticles for the construction of prostate-specific antigen biosensor
	Qu, Fei; Ding, Yanru; Lv, Xiaoxia; Xia, Lian; You, Jinmao; Han, Wenli
刊名	ANALYTICAL AND BIOANALYTICAL CHEMISTRY
	2019
卷号	411 期号:17
关键词	Fluorescence Metal-organic frameworks Gold nanoparticles Prostate-specific antigen Aptamer
英文摘要	Herein, a universal and multifunctional fluorescence sensor platform is designed by the interaction of aggregation/dispersion gold nanoparticles (AuNPs) with Tb-metal-organic frameworks (Tb-MOFs). It is found that the dispersed AuNPs rather than the aggregated ones can quench effectively the fluorescence of Tb-MOFs, and the quenching process presumably involves the mechanism of inner filter effect (IFE), dynamic quenching effect (DQE), and fluorescence resonance energy transfer (FRET). The different affinities of aptamer and aptamer-target complex toward AuNPs are employed to modulate the fluorescence signal change of Tb-MOFs. As the proof of concept, prostate-specific antigen (PSA), an efficient tumor indicator for prostate cancer, is selected as the target. At first, the PSA aptamer can protect AuNPs against salt-induced aggregation, leading to the fluorescence of Tb-MOFs quenching. Subsequently, upon PSA introduction, the rigid aptamer-PSA complex is formed and cannot stabilize AuNPs in high salt conditions, so the AuNPs aggregate significantly and the fluorescence of Tb-MOFs is restored. The linear range of PSA is achieved from 1 to 100ng/mL with a detection limit of 0.36ng/mL. Finally, this method has been validated to be sensitive and specific for PSA in human urine samples.
内容类型	期刊论文
源URL	[http://210.75.249.4/handle/363003/59758]
专题	西北高原生物研究所_中国科学院西北高原生物研究所
推荐引用方式 GB/T 7714	Qu, Fei,Ding, Yanru,Lv, Xiaoxia,et al. Emissions of terbium metal-organic frameworks modulated by dispersive/agglomerated gold nanoparticles for the construction of prostate-specific antigen biosensor[J]. ANALYTICAL AND BIOANALYTICAL CHEMISTRY,2019,411(17).
APA	Qu, Fei,Ding, Yanru,Lv, Xiaoxia,Xia, Lian,You, Jinmao,&Han, Wenli.(2019).Emissions of terbium metal-organic frameworks modulated by dispersive/agglomerated gold nanoparticles for the construction of prostate-specific antigen biosensor.ANALYTICAL AND BIOANALYTICAL CHEMISTRY,411(17).
MLA	Qu, Fei,et al."Emissions of terbium metal-organic frameworks modulated by dispersive/agglomerated gold nanoparticles for the construction of prostate-specific antigen biosensor".ANALYTICAL AND BIOANALYTICAL CHEMISTRY 411.17(2019).